A novel BAT3 sequence generated by alternative RNA splicing of exon 11B displays cell type-specific expression and impacts on subcellular localization.

PloS One
Nadine KämperNorbert Koch

Abstract

The human lymphocyte antigen (HLA) encoded BAT3/BAG6 recently attracted interest as a regulator of protein targeting and degradation, a function that could be exerted in the cytosol and in the nucleus. The BAT3 gene was described to consist of 25 exons. Diversity of transcripts can be generated by alternative RNA splicing, which may control subcellular distribution of BAT3. By cDNA sequencing we identified a novel alternatively spliced sequence of the BAT3 gene located between exons 11 and 12, which was designated as exon 11B. Using PCR and colony hybridization we identified six cDNA variants, which were produced by RNA splicing of BAT3 exons 5, 11B and 24. In four examined cell types the content of BAT3 splice variants was examined. Most of the cDNA clones from monocyte-derived dendritic cells contain exon 11B, whereas this sequence was almost absent in the B lymphoma Raji. Exon 5 was detected in most and exon 24 in approximately half of the cDNA clones. The subcellular distribution of endogenous BAT3 largely correlates with a cell type specific splicing pattern. In cells transfected with BAT3 variants, full-length and Δ24 BAT3 displayed nearly exclusive nuclear staining, whereas variants deleted of exon 11B showed substantial...Continue Reading

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Citations

Dec 7, 2013·Cellular and Molecular Life Sciences : CMLS·Janina Binici, Joachim Koch
Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Salwa SebtiSophie Pattingre
Aug 12, 2014·Bone Marrow Transplantation·I S PirasR Bacchetta
Jan 1, 2013·Journal of Biochemistry·Hiroyuki KawaharaNaoto Yokota
Feb 19, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jin-Gu Lee, Yihong Ye
Mar 19, 2014·CNS Neuroscience & Therapeutics·Zhi-Qi SongDe-Ming Zhao
Aug 16, 2016·Obesity·Dorota KaminskaJussi Pihlajamäki
Jan 8, 2020·Genome Biology and Evolution·Laramie L LindseyRobert D Bradley
Jul 16, 2014·BMC Genomics·Seong-Eui HongDo Han Kim

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Methods Mentioned

BETA
protein folding
acetylation
PCR
electrophoresis
X-ray
transfection
fluorescence microscopy
confocal microscopy
two-hybrid

Software Mentioned

ImageJ
ENSEMBL

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