A Novel Bifunctional Endolytic Alginate Lyase with Variable Alginate-Degrading Modes and Versatile Monosaccharide-Producing Properties

Frontiers in Microbiology
Chune PengFuchuan Li

Abstract

Endo-type alginate lyases usually degrade alginate completely into various size-defined unsaturated oligosaccharide products (≥disaccharides), while exoenzymes primarily produce monosaccharide products including saturated mannuronate (M) and guluronate (G) units and particularly unsaturated Δ units. Recently, two bifunctional alginate lyases have been identified as endolytic but M- and G-producing with variable action modes. However, endolytic Δ-producing alginate lyases remain undiscovered. Herein, a newFlammeovirgaprotein, Aly2, was classified into the polysaccharide lyase 7 superfamily. The recombinant enzyme and its truncated protein showed similar stable biochemical characteristics. Using different sugar chains as testing substrates, we demonstrated that the two enzymes are bifunctional while G-preferring, endolytic whereas monosaccharide-producing. Furthermore, the catalytic module of Aly2 can vary the action modes depending on the terminus type, molecular size, and M/G content of the substrate, thereby yielding different levels of M, G, and Δ units. Notably, the enzymes preferentially produce Δ units when digesting small size-defined oligosaccharide substrates, particularly the smallest substrate (unsaturated tetrasaccha...Continue Reading

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Citations

Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Jun LiuZhengqiang Jiang
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Huihui SunXiangzhao Mao
Jan 6, 2021·Applied and Environmental Microbiology·Magnus Ø ArntzenVincent G H Eijsink
Aug 4, 2020·International Journal of Biological Macromolecules·Danyang ChengXiangzhao Mao
Jun 14, 2021·Carbohydrate Polymers·Ushasree Mrudulakumari VasudevanEun Yeol Lee
Jul 14, 2021·Critical Reviews in Food Science and Nutrition·Chunhua ZhangChunpeng Wan
Feb 7, 2020·ACS Applied Materials & Interfaces·Bing WanYunlei Zhang
Aug 28, 2021·International Journal of Molecular Sciences·Fengchao ZhangWengong Yu

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Datasets Mentioned

BETA
ANQ49913.1

Methods Mentioned

BETA
electrophoresis
Assay
gel filtration
NMR
PCR
size exclusion chromatography

Software Mentioned

MEGA
Bio
Primer Premier
LipoP
BLASTp
LC solution
Promoter
SMART
Edit
ExPASy

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