A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase

BMC Biochemistry
Qiuhao QuLjubica Caldovic

Abstract

In microorganisms and plants, the first two reactions of arginine biosynthesis are catalyzed by N-acetylglutamate synthase (NAGS) and N-acetylglutamate kinase (NAGK). In mammals, NAGS produces an essential activator of carbamylphosphate synthetase I, the first enzyme of the urea cycle, and no functional NAGK homolog has been found. Unlike the other urea cycle enzymes, whose bacterial counterparts could be readily identified by their sequence conservation with arginine biosynthetic enzymes, mammalian NAGS gene was very divergent, making it the last urea cycle gene to be discovered. Limited sequence similarity between E. coli NAGS and fungal NAGK suggests that bacterial and eukaryotic NAGS, and fungal NAGK arose from the fusion of genes encoding an ancestral NAGK (argB) and an acetyltransferase. However, mammalian NAGS no longer retains any NAGK catalytic activity. We identified a novel bifunctional N-acetylglutamate synthase and kinase (NAGS-K) in the Xanthomonadales order of gamma-proteobacteria that appears to resemble this postulated primordial fusion protein. Phylogenetic analysis indicated that xanthomonad NAGS-K is more closely related to mammalian NAGS than to other bacterial NAGS. We cloned the NAGS-K gene from Xanthomon...Continue Reading

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Citations

Feb 29, 2008·Journal of Molecular Evolution·Wanessa C Lima, Carlos F M Menck
Dec 20, 2008·The Journal of Biological Chemistry·Li MinDashuang Shi
Jun 13, 2015·International Journal of Molecular Sciences·Dashuang ShiMendel Tuchman
Mar 23, 2010·Molecular Genetics and Metabolism·Ljubica CaldovicMendel Tuchman
Jan 24, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Gengxiang ZhaoDashuang Shi
Dec 25, 2012·Biochemical and Biophysical Research Communications·Gengxiang ZhaoDashuang Shi
Jul 16, 2013·Biochemical and Biophysical Research Communications·Gengxiang ZhaoDashuang Shi
Feb 9, 2021·Frontiers in Physiology·Nantaporn HaskinsLjubica Caldovic

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Datasets Mentioned

BETA
AAM41523

Methods Mentioned

BETA
acetylation
PCR

Software Mentioned

Phylip
ClustalW
Gnuplot
Protpars
Protdist

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