A novel biocatalyst, Enterobacter aerogenes LU2, for efficient production of succinic acid using whey permeate as a cost-effective carbon source

Biotechnology for Biofuels
Hubert SzczerbaZdzisław Targoński

Abstract

Succinic acid (SA), a valuable chemical compound with a broad range of industrial uses, has become a subject of global interest in recent years. The bio-based production of SA by highly efficient microbial producers from renewable feedstock is significantly important, regarding the current trend of sustainable development. In this study, a novel bacterial strain, LU2, was isolated from cow rumen and recognized as an efficient producer of SA from lactose. Proteomic and genetic identifications as well as phylogenetic analysis were performed, and strain LU2 was classified as an Enterobacter aerogenes species. The optimal conditions for SA production were 100 g/L lactose, 10 g/L yeast extract, and 20% inoculum at pH 7.0 and 34 °C. Under these conditions, approximately 51.35 g/L SA with a yield of 53% was produced when batch fermentation was conducted in a 3-L stirred bioreactor. When lactose was replaced with whey permeate, the highest SA concentration of 57.7 g/L was achieved with a yield and total productivity of 62% and 0.34 g/(L*h), respectively. The highest productivity of 0.67 g/(L*h) was observed from 48 to 72 h of batch fermentation, when E. aerogenes LU2 produced 16.23 g/L SA. This study shows that the newly isolated strai...Continue Reading

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Citations

May 1, 2021·International Journal of Molecular Sciences·Klaudia GustawAdam Waśko

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Datasets Mentioned

BETA
CAV1320
CP011574.1
EA1509E
FO203355.1
AJ110637

Methods Mentioned

BETA
genetic modification
PCR
electrophoresis

Software Mentioned

Statistica
BLAST
Image Lab
Chromax
MEGA X
MALDI Biotyper
ClustalW
FlexControl

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