A novel biosafety level 2 compliant tuberculosis infection model using a ΔleuDΔpanCD double auxotroph of Mycobacterium tuberculosis H37Rv and Galleria mellonella.

Virulence
Masanori AsaiSandra M Newton

Abstract

Mammalian infection models have contributed significantly to our understanding of the host-mycobacterial interaction, revealing potential mechanisms and targets for novel antimycobacterial therapeutics. However, the use of conventional mammalian models such as mice, are typically expensive, high maintenance, require specialized animal housing, and are ethically regulated. Furthermore, research using Mycobacterium tuberculosis (MTB), is inherently difficult as work needs to be carried out at biosafety level 3 (BSL3). The insect larvae of Galleria mellonella (greater wax moth), have become increasingly popular as an infection model, and we previously demonstrated its potential as a mycobacterial infection model using Mycobacterium bovis BCG. Here we present a novel BSL2 complaint MTB infection model using G. mellonella in combination with a bioluminescent ΔleuDΔpanCD double auxotrophic mutant of MTB H37Rv (SAMTB lux) which offers safety and practical advantages over working with wild type MTB. Our results show a SAMTB lux dose dependent survival of G. mellonella larvae and demonstrate proliferation and persistence of SAMTB lux bioluminescence over a 1 week infection time course. Histopathological analysis of G. mellonella, highli...Continue Reading

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Citations

Oct 9, 2020·Vaccines·E WhitlowS N M Hanif
Feb 27, 2021·Frontiers in Cellular and Infection Microbiology·Masanori AsaiSandra M Newton
Jun 20, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Helena EmeryChristopher J Coates

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Methods Mentioned

BETA
confocal
transmission electron microscopy
light microscopy
Confocal microscopy

Software Mentioned

Elements BR imagine
NIS
Prism

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