A novel bipartite nuclear localization signal with an atypically long linker in DOF transcription factors

Journal of Plant Physiology
Jonas KrebsSlobodan Ruzicic

Abstract

Large molecules require a nuclear localization signal (NLS) for translocation into the nucleus. Classical NLSs are rich in basic amino acids and they represent three groups, based on their structural features: SV40 T-antigen-type, yeast mating factor Matalpha-2-type, and bipartite NLSs. DNA-binding-with-one-finger (DOF) transcription factors play important roles in plants, and although their nuclear localization has been demonstrated in several cases, public protein localization prediction tools fail to detect NLS motifs in these proteins. Here, we demonstrate that an atypical bipartite NLS with a 17 amino acid long linker between its flanking basic regions directs Arabidopsis thaliana DOF proteins to the cell nucleus. The novel bipartite NLS is highly conserved in plant DOF transcription factors, including the single DOF protein in the green alga Chlamydomonas reinhardtii.

Citations

Jun 14, 2013·Plant Science : an International Journal of Experimental Plant Biology·Mélanie NogueroRichard D Thompson
Oct 7, 2014·The Plant Journal : for Cell and Molecular Biology·Lennart WirthmuellerMark J Banfield
Nov 11, 2016·Genome Génome / Conseil National De Recherches Canada·Chen DongJianghui Xie
Nov 10, 2018·FEBS Letters·Jacquelyn LaValleeKenneth W Adams
Feb 20, 2020·Journal of Experimental Botany·Begoña Renau-MorataJoaquín Medina
Oct 6, 2017·Journal of Experimental Botany·Francisco Javier Molina-HidalgoEnriqueta Moyano
Mar 14, 2019·Frontiers in Plant Science·Pilar Rojas-GraciaConcepción Gómez-Mena

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