A novel, bipartite transit peptide targets OEP75 to the outer membrane of the chloroplastic envelope

The Plant Cell
P J Tranel, K Keegstra

Abstract

OEP75 is an outer envelope membrane component of the chloroplastic protein import apparatus and is synthesized in the cytoplasm as a higher molecular weight precursor (prOEP75). During its own import, prOEP75 is processed first to an intermediate (iOEP75) and subsequently to the mature form (mOEP75). Experiments conducted with stromal extracts indicated that iOEP75 was generated from prOEP75 by the activity of the stromal processing peptidase. The specific processing site was determined and used to divide the prOEP75 transit peptide into N- and C-terminal domains. To determine the targeting functions of the two domains of the transit peptide and of the mature region of prOEP75, we created a deletion mutant construct from prOEP75 and chimeric constructs between domains of prOEP75 and the precursor to a small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Analysis of these constructs by in vitro chloroplastic protein import assays revealed that the transit peptide of prOEP75 is bipartite in that the N- and C-terminal portions contain chloroplastic and intraorganellar targeting information, respectively.

References

Feb 26, 1990·FEBS Letters·Y Gavel, G von Heijne
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M SalomonJ Soll
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Mar 1, 1995·The Plant Journal : for Cell and Molecular Biology·M SeedorfJ Soll
Jan 15, 1995·European Journal of Biochemistry·G C ShoreM Nguyen
Oct 1, 1994·Plant Molecular Biology·C Robinson, R B Klösgen
Feb 1, 1996·Trends in Biochemical Sciences·N PfannerG C Shore

❮ Previous
Next ❯

Citations

Apr 29, 2009·Cellular and Molecular Life Sciences : CMLS·Dirk M WaltherJan Tommassen
Jun 15, 2007·Photosynthesis Research·Maryse A BlockNorbert Rolland
Dec 23, 1999·Current Opinion in Plant Biology·G I McFadden
Jan 25, 2005·Plant Biology·T BeckerE Schleiff
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Maik S SommerEnrico Schleiff
May 24, 2005·Plant Physiology·Amy BaldwinPaul Jarvis
Mar 23, 2002·The Plant Cell·Xuejun ChenDanny J Schnell
Apr 24, 1999·The Plant Cell·K Keegstra, K Cline
Oct 13, 2000·Biological Chemistry·N SveshnikovaE Schleiff
Nov 15, 2000·Biological Chemistry·U C Vothknecht, J Soll
Jan 5, 2011·Biological Chemistry·Enrico SchleiffThomas Becker
Nov 11, 2008·Bioscience, Biotechnology, and Biochemistry·R M Udayangani RatnayakeMitsuru Akita
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S ReumannK Keegstra
Mar 3, 2010·Annual Review of Plant Biology·Hsou-min Li, Chi-Chou Chiu
Aug 12, 2005·Molecular Membrane Biology·Sigrun ReumannKenneth Keegstra
Nov 9, 2005·The Journal of Cell Biology·Kentaro InoueMasaru Ohme-Takagi
Oct 25, 2006·The Journal of Cell Biology·Ming Li, Danny J Schnell
Aug 3, 2006·Cortex; a Journal Devoted to the Study of the Nervous System and Behavior·Julie A FiezHanna Damasio
Nov 3, 2005·EMBO Reports·Enrico Schleiff, Jürgen Soll
Dec 17, 2008·The New Phytologist·Paul Jarvis
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology·Nancy R Hofmann, Steven M Theg
Aug 16, 2011·The Plant Journal : for Cell and Molecular Biology·John E Froehlich, Kenneth Keegstra
Jun 23, 2011·Chembiochem : a European Journal of Chemical Biology·Bettina Bölter, Jürgen Soll
Aug 30, 2005·Trends in Biochemical Sciences·Stefan A PaschenDoron Rapaport
Aug 9, 2005·Trends in Plant Science·Nancy R Hofmann, Steven M Theg
Mar 28, 2012·Biochimica Et Biophysica Acta·Dong Wook LeeInhwan Hwang
Dec 26, 2001·Biochimica Et Biophysica Acta·E Schleiff, R B Klösgen
Oct 16, 2012·Biochimica Et Biophysica Acta·Lan-Xin Shi, Steven M Theg
Jan 19, 2015·International Journal of Medical Microbiology : IJMM·Thomas Ulrich, Doron Rapaport

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.