DOI: 10.1101/519082Jan 14, 2019Paper

A novel blaSIM-1-carrying megaplasmid pSIM-1-BJ01 isolated from clinical Klebsiella pneumonia

BioRxiv : the Preprint Server for Biology
Yang LüHongyan Hu

Abstract

A rare carbapenem-resistant gene bla SIM-1 was found in a 316-kb megaplasmid designated pSIM-1-BJ01 isolated from a clinical strain Klebsiella pneumonia 13624. The plasmid pSIM-1-BJ01 was fully sequenced and analyzed. Its length is 316,557 bp and it has 342 putative open reading frames with two multidrug-resistant regions and a total of 19 resistant genes. Its backbone was highly homologous to the newly reported plasmid pRJA166a, which was isolated from a clinical third-generation cephalosporin-resistant hypervirulen strain K. pneumonia ST23. The plasmid pSIM-1-BJ01 was verified to be able to transfer to Escherichia coli . The emergency of the transferable bla SIM-1-carrying multidrug-resistant plasmid pSIM-1-BJ01 suggests the spread of bla SIM among Enterobacteriaceae is possible. Therefore, the data presented herein provided insights into the genomic diversity and evolution of bla SIM-carrying plasmids, as well as the dissemination and epidemiology of bla SIM among Enterobacteriaceae in public health system.

Related Concepts

Klebsiella pneumoniae
Carbapenems
Enterobacteriaceae
Epidemiology
Escherichia coli
Biological Evolution
Genes
Genome
Plasmids
Disseminated

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.