A novel bronchial ring bioassay for the evaluation of small airway smooth muscle function in mice

American Journal of Physiology. Lung Cellular and Molecular Physiology
John Q LiuRodney J Folz

Abstract

Advances in our understanding of murine airway physiology have been hindered by the lack of suitable, ex vivo, small airway bioassay systems. In this study, we introduce a novel small murine airway bioassay system that permits the physiological and pharmacological study of intrapulmonary bronchial smooth muscle via a bronchial ring (BR) preparation utilizing BR segments as small as 200 microm in diameter. Using this ex vivo BR bioassay, we characterized small airway smooth muscle contraction and relaxation in the presence and absence of bronchial epithelium. In control BRs, the application of mechanical stretch is followed by spontaneous bronchial smooth muscle relaxation. BRs pretreated with methacholine (MCh) partially attenuate this stretch-induced relaxation by as much as 42% compared with control. MCh elicited a dose-dependent bronchial constriction with a maximal tension (E(max)) of 8.7 +/- 0.2 mN at an EC(50) of 0.33 +/- 0.02 microM. In the presence of nifedipine, ryanodine, 2-aminoethoxydiphenyl borate, and SKF-96365, E(max) to MCh was significantly reduced. In epithelium-denuded BRs, MCh-induced contraction was significantly enhanced to 11.4 +/- 1.0 mN with an EC(50) of 0.16 +/- 0.04 microM (P < 0.01). Substance P rela...Continue Reading

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Citations

Dec 19, 2009·The Journal of Biological Chemistry·Wen-Cheng ZhangMin-Sheng Zhu
Aug 10, 2010·The European Respiratory Journal·F LiK F Chung
Mar 9, 2013·PLoS Biology·Cheng-Hai ZhangRonghua ZhuGe
Mar 1, 2012·American Journal of Veterinary Research·Anne A WooldridgeSharron R Barney
Apr 10, 2007·The Journal of Allergy and Clinical Immunology·Pascal ChanezIsabelle Vachier
Apr 24, 2015·Science Translational Medicine·Polina L YarovaDaniela Riccardi
May 8, 2020·Molecular Medicine Reports·Jing ChenHe-Zuo Lü
Nov 9, 2011·The Journal of Pharmacology and Experimental Therapeutics·Samuel J TaylorPeter J Henry
Feb 6, 2020·Journal of the Endocrine Society·Emilie Balk-MøllerHannelouise Kissow
Jan 28, 2021·Journal of Ethnopharmacology·Kátia Simoni Bezerra LimaLarissa Araújo Rolim
Jun 1, 2021·Journal of Ethnopharmacology·Pedro Modesto Nascimento MenezesLarissa Araújo Rolim

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