A novel calmodulin-β-PIX interaction and its implication in receptor tyrosine kinase regulation

Cellular Signalling
Vinay K SinghZongchao Jia

Abstract

Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates numerous cellular processes, primarily in response to calcium flux. We have identified and characterized a novel interaction between CaM and β-p21-activated kinase interacting exchange factor (β-PIX), a putative guanine exchange factor implicated in cell signaling, using affinity pull-down assays, co-immunoprecipitation, co-localization and circular dichroism studies. Fluorescence-based titration and isothermal titration calorimetry experiments revealed a Ca(2+)-dependent binding mechanism (K(D)≤10μM). Further, we show that CaM participates in a multi-protein complex involving β-PIX and E3 ubiquitin ligase c-Cbl (casitas B-cell lymphoma), which may play a critical role in receptor tyrosine kinase regulation and downstream signaling.

References

Apr 18, 1980·Science·J L Marx
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C Y HuangR K Sharma
Jun 15, 1994·Molecular and Cellular Biochemistry·M P Walsh
Aug 12, 1999·Cellular and Molecular Life Sciences : CMLS·R KakkarR K Sharma
Sep 22, 2001·Endocrine-related Cancer·E ZwickA Ullrich
Aug 15, 2002·The Journal of Biological Chemistry·Iwona SzymkiewiczIvan Dikic
Mar 22, 2003·Current Opinion in Cell Biology·Ivan Dikic, Silvia Giordano
Jun 13, 2003·Oncogene·Lorenza PenengoGiovanni Gaudino
Sep 25, 2003·Cell·Wen Jin WuRichard A Cerione
Sep 25, 2003·The Journal of Biological Chemistry·Shine TuRichard A Cerione
Oct 3, 2003·Cellular and Molecular Life Sciences : CMLS·I DikicP Soubeyran
Dec 4, 2003·Biochemical Society Transactions·I Dikic
Dec 13, 2003·Endocrine Reviews·Christina R Kahl, Anthony R Means
Apr 15, 2004·The Biochemical Journal·Hongbing LiAntonio Villalobo
Jul 2, 2004·The EMBO Journal·Kristi G BacheHarald Stenmark
Dec 22, 2004·The Journal of Biological Chemistry·Georg RosenbergerKerstin Kutsche
Dec 29, 2004·Trends in Endocrinology and Metabolism : TEM·Tim D WerryArthur Christopoulos
Apr 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xinchun ShenRihe Liu
Sep 9, 2005·The EMBO Journal·Kaisa Haglund, Ivan Dikic
Oct 18, 2005·Nature Structural & Molecular Biology·Daniela JozicJerónimo Bravo
Dec 13, 2005·European Journal of Cell Biology·Georg Rosenberger, Kerstin Kutsche
Apr 4, 2006·Cell Cycle·Carola Reindl, Karsten Spiekermann
Dec 26, 2006·Molecular Cancer Research : MCR·Nicola CrosettoIvan Dikic

❮ Previous
Next ❯

Citations

Nov 6, 2013·Biochimica Et Biophysica Acta·Martin W Berchtold, Antonio Villalobo
Mar 31, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vadym SulimenkoPavel Dráber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.