A novel candidate tumour suppressor locus at 9q32-33 in bladder cancer: localization of the candidate region within a single 840 kb YAC

Human Molecular Genetics
T HabuchiM A Knowles

Abstract

Loss of heterozygosity (LOH) on chromosome 9q is the most frequent genetic alteration in transitional cell carcinoma (TCC) of the bladder, implicating the presence of a tumour suppressor gene or genes on 9q. To define the location of a tumour suppressor locus on 9q in TCC, we screened 156 TCCs of the bladder and upper urinary tract by detailed deletion mapping using 31 microsatellite markers on 9q. Partial deletions of 9q were found in 10 TCCs (6%), and LOH at all informative loci on 9q was found in 77 TCCs (49%). In five low grade superficial bladder tumours, the partial deletion was localized to D9S195 located at 9q32-33, with retention of heterozygosity at all other informative loci including D9S103, D9S258, D9S275 and GSN. We constructed a yeast artificial chromosome (YAC) contig covering the deleted region in these five tumours and placed another four unmapped microsatellite markers on this contig map. Using these markers, we further defined the common deleted region to the interval between D9S1848 and AFMA239XA9. The region is covered by a single YAC (852e11), whose size is estimated to be 840 kb. Our data should expedite further fine mapping and identification of the candidate tumour suppressor gene at 9q32-33.

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Citations

Mar 29, 2002·Genes, Chromosomes & Cancer·Sarah V WilliamsMargaret A Knowles
Oct 11, 2002·The Journal of Pathology·Angela A G van TilborgEllen C Zwarthoff
May 1, 2001·The Journal of Pathology·A A van TilborgE C Zwarthoff
Aug 16, 2008·International Journal of Clinical Oncology·Margaret A Knowles
Apr 29, 2005·Brain Research. Molecular Brain Research·Toshiyuki NakataniIchiro Matsuoka
Mar 21, 2003·Cancer Genetics and Cytogenetics·Helen Therese Moriarty, Lucy Robyn Webster
Jul 19, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J LouhelainenK Hemminki
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Mar 26, 2010·Expert Reviews in Molecular Medicine·Courtney PollardDan Theodorescu
Feb 12, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Kirsten GrønbaekPer Guldberg
Jul 31, 2001·Molecular Pathology : MP·M A Knowles
Dec 5, 2008·Scandinavian Journal of Urology and Nephrology. Supplementum·Margaret A Knowles
Mar 24, 2009·The Journal of Urology·Karen L PriceDavid A Long
Feb 20, 2007·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Shan GaoJens Ahm Sørensen
Jan 13, 2011·The American Journal of Pathology·Francesca ZammarchiChiara Maria Mazzanti
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Sep 17, 1999·The American Journal of Pathology·S KnuutilaY Zhu
Apr 10, 1999·Electrophoresis·M A Knowles
Dec 24, 2004·Journal of Dental Research·E Dabelsteen, S Gao
Jan 9, 2004·Oncogene·Kate O WrightJay E Reeder
Jun 3, 1999·Oncogene·N HornigoldM A Knowles
May 10, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yoshitomo ChiharaYoshihiko Hirao
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Sep 27, 2014·Molecular Biology Reports·Knud LarsenChristian Bendixen
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