A novel canine histiocytic sarcoma cell line: initial characterization and utilization for drug screening studies

BMC Cancer
Marilia TakadaVilma Yuzbasiyan-Gurkan

Abstract

Histiocytic sarcoma is a rare disorder in humans, however it is seen with appreciable frequency in certain breeds of dogs, such as Bernese mountain dog. The purpose of this study was to fully characterize a novel canine histiocytic sarcoma cell line, and utilize it as a tool to screen for potential therapeutic drugs. The histiocytic sarcoma cell line was characterized by expression of cellular markers as determined by immunohistochemistry and flow cytometry techniques. The neoplastic cells were also evaluated for their capability of phagocytizing beads particles, and their potential to grow as xenograft in an immunodeficient mouse. We investigated the in vitro cytotoxic activity of a panel of thirteen compounds using the MTS proliferation assay. Inhibitory effects of different drugs were compared using one-way ANOVA, and multiple means were compared using Tukey's test. Neoplastic cells expressed CD11c, CD14, CD18, CD45, CD172a, CD204, MHC I, and vimentin. Expression of MHC II was upregulated after exposure to LPS. Furthermore, the established cell line clearly demonstrated phagocytic activity similar to positive controls of macrophage cell line. The xenograft mouse developed a palpable subcutaneous soft tissue mass after 29 day...Continue Reading

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Citations

Sep 22, 2018·Oncotarget·Hunter John PiegolsVilma Yuzbasiyan-Gurkan
Jul 7, 2019·Genes·Marilia TakadaVilma Yuzbasiyan-Gurkan
Aug 24, 2018·Molecular Cancer Therapeutics·Marilia TakadaVilma Yuzbasiyan-Gurkan

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Methods Mentioned

BETA
genotyping
xenograft
Assay
flow cytometry

Software Mentioned

Image [UNK] Lite
GraphPad
GraphPad Prism

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