A novel cannulation technique for isolation of human hepatocytes from explanted diseased whole livers

Transplantation Proceedings
D C KehrIgor M Sauer

Abstract

Diseased human organs explanted during liver transplantation can be used as a cell source for basic research and future therapeutic applications in regenerative medicine. Enzymatic digestion using the perfusion technique has become the gold standard in liver cell isolation. Usually the portal vein is used as a vascular access for liver cell isolation from explanted livers, that were rejected from whole organ transplantation. No special techniques are required for cannulation; the cannulas are simply introduced into the vessels and a ligature is then thrown around the vessel to secure the cannulation. This method is not applicable to organs explanted during liver transplant surgery, because as much of the vessels as possible has to be kept in situ, to facilitate anastomosis of the new organ. Therefore, when perfusing the explanted organ, normal perfusion catheters are easily displaced and a more complex "vascular reconstruction" must be performed to secure hold of the catheters. We established a novel cannulation technique using commercially available Foley catheters for liver cell isolation from diseased whole organs explanted during transplant surgery. We evaluated this technique in 15 diseased organs. 5 were isolated in the c...Continue Reading

References

Jun 1, 1997·Transplantation Proceedings·W R HewittJ Rozga
Aug 23, 2001·The Journal of Surgical Research·J C GerlachP Neuhaus
Mar 11, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Robin D HughesPaolo Muiesan
Jul 24, 2007·Cells, Tissues, Organs·J LaursonH J F Hodgson
Aug 3, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Toshiyasu KawaharaNorman M Kneteman

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Citations

Jul 6, 2014·Seminars in Pediatric Surgery·Joseph P Vacanti, Katherine M Kulig

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