A novel carbon fiber bundle microelectrode and modified brain slice chamber for recording long-term multiunit activity from brain slices

Journal of Neuroscience Methods
T K Tcheng, M U Gillette

Abstract

The fabrication and characteristics of a novel multiunit recording electrode and modified brain slice chamber suitable for long-term recording from brain slices are described. The electrode consisted of an electrolyte-filled glass micropipette with a 20-50 microns thick wax-coated bundle of 5-micron diameter carbon fibers extending 2.5 cm from the tapered end and an AgCl-coated silver wire inserted into the open end and connected to a preamplifier. Both ends of the electrode were sealed with wax to prevent evaporation of the electrolyte. The brain slice was maintained over this extended period in an interface-type brain slice chamber modified to completely surround the slice with medium. Using this electrode, regular 24-h oscillations of spontaneous multiunit activity were recorded for 3 days from a single location in a 500 microns thick rat suprachiasmatic nucleus brain slice. Preliminary data suggest that this novel carbon fiber bundle electrode will be a favorable alternative to traditional metal electrodes for long-term recording of multiunit activity from brain slices.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S T Inouye, H Kawamura
Dec 1, 1979·Journal of Neuroscience Methods·H L HaasM Vosmansky
Oct 1, 1969·Journal of Pharmaceutical Sciences·R N Adams
Jun 15, 1980·The Journal of Comparative Neurology·A N Van den Pol
Jul 1, 1980·Brain Research Bulletin·G I HattonC D Tweedle
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y Bouskila, F E Dudek

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Citations

Jun 17, 2014·Scientific Reports·Yossi BuskilaJohn W Morley
Nov 16, 2010·Journal of Neuroscience Methods·M R H Hill, S A Greenfield
Sep 27, 2014·Journal of Neuroscience Methods·David M DorrisJohn Meitzen
Feb 22, 2017·The Journal of Physiology·Lydia HannaTimothy M Brown
Mar 22, 2000·Nature Neuroscience·A JagotaW J Schwartz
Apr 6, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M MrugalaW J Schwartz

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