A Novel Cecropin-LL37 Hybrid Peptide Protects Mice Against EHEC Infection-Mediated Changes in Gut Microbiota, Intestinal Inflammation, and Impairment of Mucosal Barrier Functions.

Frontiers in Immunology
Xubiao WeiManyi Zhang

Abstract

Intestinal inflammation can cause impaired epithelial barrier function and disrupt immune homeostasis, which increases the risks of developing many highly fatal diseases. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 causes intestinal infections worldwide and is a major pathogen that induces intestinal inflammation. Various antibacterial peptides have been described as having the potential to suppress and treat pathogen-induced intestinal inflammation. Cecropin A (1-8)-LL37 (17-30) (C-L), a novel hybrid peptide designed in our laboratory that combines the active center of C with the core functional region of L, shows superior antibacterial properties and minimized cytotoxicity compared to its parental peptides. Herein, to examine whether C-L could inhibit pathogen-induced intestinal inflammation, we investigated the anti-inflammatory effects of C-L in EHEC O157:H7-infected mice. C-L treatment improved the microbiota composition and microbial community balance in mouse intestines. The hybrid peptide exhibited improved anti-inflammatory effects than did the antibiotic, enrofloxacin. Hybrid peptide treated infected mice demonstrated reduced clinical signs of inflammation, reduced weight loss, reduced expression of pro-inflamma...Continue Reading

References

Aug 6, 1999·Immunity·T KawaiS Akira
Oct 30, 2001·Digestive Diseases and Sciences·S ChoudharyA Banan
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Jun 13, 2002·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·H DosogneC Burvenich
Sep 20, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·I L KhalifI D Maximova
Jul 5, 2006·Nature Clinical Practice. Gastroenterology & Hepatology·R Balfour Sartor
Jun 15, 2007·The American Journal of Gastroenterology·Steven J Brown, Lloyd Mayer
Feb 5, 2008·Gastroenterology·R Balfour Sartor
Jul 25, 2008·Nature·Ruslan Medzhitov
Nov 10, 2009·Digestive Diseases·Nina A Hering, Jörg-Dieter Schulzke
Dec 18, 2009·Mucosal Immunology·M Wlodarska, B B Finlay
Feb 2, 2010·Journal of Pediatric Gastroenterology and Nutrition·Eek J ParkMichael T Clandinin
Mar 18, 2011·Scandinavian Journal of Gastroenterology·In-Ah Lee, Dong-Hyun Kim
Jun 11, 2011·The Journal of Nutritional Biochemistry·Erica L M VieiraJacqueline I Alvarez-Leite
May 1, 2013·Nature Reviews. Drug Discovery·Kim Lewis
Dec 11, 2013·The American Journal of Gastroenterology·Maria PierdomenicoSalvatore Cucchiara
Feb 15, 2014·BMC Gastroenterology·Terry SigmanErnest G Seidman
Apr 25, 2014·Nature Reviews. Genetics·Darren J Burgess
Oct 17, 2014·Archives of Insect Biochemistry and Physiology·Eunjung LeeYangmee Kim
Dec 4, 2014·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·H Sales-CamposC R B Cardoso
Jan 17, 2015·Nature·Manolis Pasparakis, Peter Vandenabeele
Jan 21, 2015·The Turkish Journal of Gastroenterology : the Official Journal of Turkish Society of Gastroenterology·Camelia CojocariuAnca Trifan
Jan 21, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Feifei HanYizhen Wang
Feb 18, 2015·Veterinary Research Communications·Chrząstek Klaudia, Wieliczko Alina
Jun 14, 2015·The Journal of Nutritional Biochemistry·Geoffrey A PreidisJoseph F Petrosino
Nov 28, 2015·Mediators of Inflammation·Yue Sun, Dejing Shang
Jul 2, 2016·International Journal of Molecular Sciences·Xu-Biao WeiRi-Jun Zhang
Jan 27, 2017·Drug and Chemical Toxicology·Małgorzata Pomorska-MólZygmunt Pejsak
Sep 10, 2018·The Journal of Nutritional Biochemistry·Suprabhat MukherjeeSanti P Sinha Babu
Dec 14, 2018·International Journal of Molecular Sciences·Valéria Costa da SilvaGerlane Coelho Bernardo Guerra

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Methods Mentioned

BETA
Assay
enzyme-linked immunosorbent assay
ELISA
electrophoresis
Illumina sequencing

Software Mentioned

GraphPad Prism
UPARSE

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