A novel cell-free strategy for promoting mouse liver regeneration: utilization of a conditioned medium from adipose-derived stem cells

Hepatology International
Sang Kuon LeeSay-June Kim

Abstract

Although stem cells have beneficial effects, their clinical application faces many issues, including high cost and safety. Because stem cell plasty is largely based on their paracrine activity, this study aimed to test the hypothesis that utilization of the stem-cell secretome instead of actual cells would not only overcome these limitations, but also have similar effects as stem cell-based therapy. Partial hepatectomized mice were divided into four groups according to the material administered via the tail vein: normal saline (saline group); 1.0 × 10(6) human adipose tissue-derived stem cells (ASCs) in 0.1 mL saline (ASC group); 25-fold concentrated conditioned medium from ASCs (ASC-secretome group); and concentrated medium (media group). Specimens were obtained postoperatively. Liver regeneration was estimated by bromodeoxyuridine incorporation, Lgr5 RT-PCR, proliferating cell nuclear antigen western blot, and liver weights, and liver function was estimated by albumin immunohistochemistry and liver function tests. The liver regenerative capacities of the ASC and ASC-secretome groups were not statistically different from each other, but were higher than their respective control groups. Moreover, the ASC and ASC-secretome group...Continue Reading

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Citations

Aug 16, 2016·Stem Cells and Development·Debora Lo FurnoRosario Giuffrida
Nov 9, 2017·Frontiers in Pharmacology·Majid AlhomraniWilliam Sievert
Jan 8, 2019·The Laryngoscope·Choung-Soo KimDong-Il Sun
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Nov 25, 2019·Journal of Korean Medical Science·Jung Hyun ParkSay June Kim
Oct 5, 2019·Current Medicinal Chemistry·Anjum MahmoodAnand S Srivastava

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