PMID: 8995666Feb 1, 1997Paper

A novel cellular site-specific DNA-binding protein cooperates with the viral NS1 polypeptide to initiate parvovirus DNA replication

Journal of Virology
J ChristensenP Tattersall

Abstract

Replication of linear single-stranded parvovirus DNA proceeds by a rolling-hairpin mechanism which generates long, palindromic, duplex concatamers. Processing to monomer length requires initiation from origins of DNA replication located at the 3' and 5' ends of each embedded monomer, reactions which can be recapitulated in vitro for minute virus of mice (MVM). To determine which cellular proteins were essential for replication from these origins, S100 extracts from 293S cells were fractionated on phosphocellulose. When recombined, these fractions were able to support replication in vitro, dependent on the viral initiator protein NS1, using plasmid forms of the 5' origin or the minimal 3' origin as templates. Fraction P-cell 1 contains two factors, replication protein A (RPA) and proliferating-cell nuclear antigen (PCNA), known to be essential for simian virus 40 replication in vitro. When P-cell 1 was replaced with purified recombinant RPA and PCNA, NS1-mediated MVM replication initiated from the 5' origin but not from the 3' origin. The 3' origin is a 50-bp sequence containing three distinct recognition elements, an NS1 binding site, a site at which NS1 nicks the DNA to generate the priming 3' OH, and a region containing a con...Continue Reading

References

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Citations

May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·T BashirK Willwand
May 14, 2003·Journal of Virology·Susan F Cotmore, Peter Tattersall
Aug 30, 2013·Journal of Virology·Einari A NiskanenMaija Vihinen-Ranta
Jul 5, 2002·Clinical Microbiology Reviews·Erik D Heegaard, Kevin E Brown
Mar 12, 2010·Journal of Virology·Einari A NiskanenMaija Vihinen-Ranta
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·K QingA Srivastava
Oct 17, 2001·Journal of Virology·A Op De BeeckP Caillet-Fauquet
Feb 1, 2013·Journal of Virology·Yong LuoJianming Qiu
Apr 11, 2007·Cellular Microbiology·Teemu O IhalainenMaija Vihinen-Ranta
Nov 26, 1997·Journal of Virology·J CorsiniE Winocour
Jul 6, 2021·Frontiers in Microbiology·Liting ShaoJianming Qiu
Jun 14, 2008·European Journal of Internal Medicine·Spyros AslanidisChryssanthos Zamboulis

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