PMID: 18187864Jan 12, 2008Paper

A novel chip device based on wired capillary packed with high performance polymer-based monolith for HPLC: reproducibility in preparation processes to obtain long columns

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Ken HosoyaNobuo Tanaka

Abstract

This report describes the development of novel wired chip devices for mu-HPLC analyses. The monolithic capillary column to be wired was prepared using a tri-functional epoxy monomer, tris(2,3-epoxypropyl)isocyanurate with a diamine, 4-[(4-aminocyclohexyl)methyl]cyclohexylamine. The prepared column was evaluated by SEM observation of the sectional structure of column and micro-HPLC. In addition, the reproducibility in the preparation of long capillary columns having nearly 1 m length was extensively examined for applications of novel wired chip devices. The authors demonstrated that the monolithic structure of the prepared long capillary could be finely controlled under the strictly maintained operational conditions and thus the relative standard deviation (RSD) of the column properties such as the number of theoretical plates, retention factor, and permeability could be well controlled to become less than 10%. Furthermore, the wired chip device column showed that its high performance was kept even after chip preparation.

References

Dec 17, 2003·Analytical Chemistry·Chengdu LiangGeorges Guiochon
Jan 5, 2005·Journal of Chromatography. a·H A Claessens, M A van Straten
Mar 15, 2005·Analytical Chemistry·Masaru KatoToshimasa Toyo'oka
Mar 24, 2005·Journal of Chromatography. a·Galina A Platonova, Tatiana B Tennikova
May 10, 2005·Mutation Research·L Mark Hewitt, Chris H Marvin
Jun 9, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroshi KobayashiNobuo Tanaka
Aug 16, 2006·Analytical Chemistry·Ken HosoyaNobuo Tanaka
Jul 13, 2007·Journal of Chromatography. a·Kai-Feng DuYan Sun

❮ Previous
Next ❯

Citations

Sep 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tomohiko HiranoHajime Ohtani
Mar 11, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Tomoko MoriKen Hosoya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.