A novel chloroplast localized Rab GTPase protein CPRabA5e is involved in stress, development, thylakoid biogenesis and vesicle transport in Arabidopsis

Plant Molecular Biology
Sazzad KarimHenrik Aronsson

Abstract

A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloroplasts (stroma and thylakoids). Ypt31/32 in the yeast Saccharomyces cerevisiae are involved in regulating vesicle transport, and CPRabA5e a close homolog of Ypt31/32, restores the growth of the ypt31Δ ypt32(ts) mutant at 37 °C in yeast complementation. Knockout mutants of CPRabA5e displayed delayed seed germination and growth arrest during oxidative stress. Ultrastructural studies revealed that after preincubation at 4 °C mutant chloroplasts contained larger plastoglobules, lower grana, and more vesicles close to the envelopes compared to wild type, and vesicle formation being enhanced under oxidative stress. This indicated altered thylakoid development and organization of the mutants. A yeast-two-hybrid screen with CPRabA5e as bait revealed 13 interacting partner proteins, mainly located in thylakoids and plastoglobules. These proteins are known or predicted to be involved in development, stress responses, and photos...Continue Reading

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Citations

Dec 9, 2014·Plant Signaling & Behavior·Emelie Lindquist, Henrik Aronsson
Jun 17, 2014·Traffic·Anna K HurlockChristoph Benning
Feb 11, 2015·Biochimica Et Biophysica Acta·Barry J PogsonVerónica Albrecht-Borth
Jan 24, 2015·Biochimica Et Biophysica Acta·Anna RastJörg Nickelsen
Oct 9, 2014·Frontiers in Plant Science·Sazzad Karim, Henrik Aronsson
Jul 15, 2016·Traffic·Emelie LindquistHenrik Aronsson
Feb 6, 2017·International Journal of Molecular Sciences·Congfei YinHenrik Aronsson
Aug 18, 2018·Photosynthesis Research·Emelie Lindquist, Henrik Aronsson
Dec 14, 2018·Frontiers in Plant Science·Xiaomin LiuFei Bao
Oct 17, 2019·International Journal of Molecular Sciences·Ping GanJijing Luo
Apr 9, 2019·Open Biology·Annabel MechelaJürgen Soll
Apr 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Alexander P HertleKrishna K Niyogi
Apr 21, 2021·Journal of Experimental Botany·Ana K Machado WoodKostya Kanyuka

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