Abstract
Background: Breast cancer (BC) represents a heterogeneous disease with distinct subtypes and high tumor metastatic potentials. Recent researchers identify the implication of circular RNAs (circRNAs) in the initiation of BC. Herein, we uncover a novel circRNA hsa_circRPPH1_015 as a tumor promoter in BC. Methods: A total of 86 paired cancerous and non-cancerous tissues were surgically resected and collected from BC patients. Cell proliferation, colony formation, and cell invasion were examined by Edu staining, clone formation assays, propidium iodide (PI)-labeled flow cytometry, and Transwell invasion assays. PCNA, Ki67, MMP-2, MMP-9, Cyclin D1, and CDK4 expression was assayed using Western blot analysis. RNA pull-down, dual-luciferase reporter gene assay, and RNA binding protein immunoprecipitation (RIP) assay were performed to investigate the relationships among hsa_circRPPH1_015, microRNA-326 (miR-326), and ELK1. The tumor growth of human BC MCF-7 cells in vivo was evaluated in nude mice by subcutaneous xenografts of MCF-7 cells. Results: hsa_circRPPH1_015 expression was upregulated in BC tissues. Knockdown of hsa_circRPPH1_015 restrained the aggressive behavior of MCF-7. hsa_circRPPH1_015 could bind to miRNA-326 that negative...Continue Reading
References
May 29, 2010·BMC Systems Biology·Zhiao ShiBing Zhang
Jun 12, 2013·Nucleic Acids Research·Mickael LeclercqMathieu Blanchette
May 30, 2014·The Lancet Oncology·Lei FanPaul E Goss
Jul 12, 2014·Nature Reviews. Drug Discovery·Zhonghan Li, Tariq M Rana
Sep 6, 2015·Oncotarget·Takashi KawaharaHiroshi Miyamoto
Apr 5, 2016·OncoTargets and Therapy·Qiuyue YanXuejun Dong
May 10, 2016·International Journal of Biological Macromolecules·A AzevedoR F Gerlach
Oct 14, 2016·Oncology Research·Sheqing JiYawei Hua
Nov 21, 2016·Lancet·Nadia Harbeck, Michael Gnant
Dec 28, 2016·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Chun-Yu LiuKuen-Feng Chen
Feb 6, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kyle J IsaacsonHamidreza Ghandehari
May 23, 2017·Oncology Letters·Ping ZhangLin Zhu
Aug 16, 2017·Molecular Cancer·Hui WangWenxin Qin
Sep 22, 2017·DNA and Cell Biology·Yi-Yin TangXiao-Li Wang
Oct 3, 2017·Annals of Diagnostic Pathology·Mohamad Nidal KhabazJaudah Al-Maghrabi
Oct 24, 2017·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·C YangJ Zheng
Nov 9, 2017·International Journal of Oncology·Jiandong WangQiugen Wang
Mar 30, 2018·Cancer Management and Research·Juan ZhouSan-Gang Wu
Apr 18, 2018·Journal of Cellular Physiology·Ren WangXiao-Yan Jin
Apr 21, 2018·The Breast : Official Journal of the European Society of Mastology·An De GroefNele Devoogdt
May 10, 2018·BMC Genomics·Shobana SekarWinnie S Liang
Jun 13, 2018·Genes & Genomics·Akhlaq AhmadTao Zhu
Sep 13, 2018·CA: a Cancer Journal for Clinicians·Freddie BrayAhmedin Jemal
Sep 22, 2018·OncoTargets and Therapy·Wanjun YuJipeng Li
Sep 23, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xuan LiangTie Chong
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·He-da ZhangJin-Hai Tang
Oct 6, 2018·European Journal of Breast Health·Aylin ErdimA Özdemir Aktan
Dec 7, 2018·Molecular Carcinogenesis·Danfeng GaoLihua Li
Dec 7, 2018·Cell Cycle·Jianzhu ZhaoJun Tang
Jan 10, 2019·Molecular Cancer·Rui YangJunxia Chen
Jan 11, 2019·Molecular Cancer·Qingfeng ShangShengfang Ge
Jan 23, 2019·JAMA : the Journal of the American Medical Association·Adrienne G Waks, Eric P Winer
Jan 28, 2019·Cancer Treatment Reviews·Stefano FogliRomano Danesi
Jul 18, 2019·Bioscience Reports·Ye DuHaipeng Zhang
Jul 25, 2019·Translational Oncology·Yan WangLiuxing Wang