PMID: 7523117Sep 15, 1994Paper

A novel class of adhesion acidic glycans in sea urchin embryos. Isolation, characterization and immunological studies during early embryonal development

European Journal of Biochemistry
E PapakonstantinouG Misevic

Abstract

Total glycans were isolated and purified from Lytechinus pictus embryos at early developmental stages by gel-filtration chromatography after pronase and DNase digestion, and alkali-borohydride treatment. Fractionation by Superose 6 and HPLC gel-filtration chromatography revealed three major glycan fractions of 580, 150 and 2 kDa consistently throughout development up to the stage of end gastrula. The 580-kDa and the 150-kDa glycan fractions isolated from fertilized eggs up to the stage of end gastrula are highly acidic, whereas the 2-kDa glycan fractions have no detectable uronic acid residues and charged groups. Chemical analysis of the glycan fractions showed that their content of neutral hexoses, uronic acid, GlcNAc, GalNAc and sulphate changes during development. The resistance of the 580-kDa and the 150-kDa glycan fractions to glycosaminoglycan-degrading enzymes indicates a structure which is different from the glycosaminoglycans. The incorporation of [3H]glucosamine into the 580-kDa, the 150-kDa and the 2-kDa glycan fractions showed that glycan synthesis increases in a linear fashion from the stage of early blastulation to end of gastrulation. Maximal incorporation of the radioligand occurs in the 2-kDa glycan fractions, ...Continue Reading

References

Aug 28, 1990·European Journal of Biochemistry·K K KaramanosC P Tsiganos
Aug 1, 1974·Experimental Cell Research·S Kinoshita
Dec 7, 1984·Journal of Chromatography·B A BidlingmeyerT L Tarvin
Jun 1, 1983·Experimental Cell Research·K Akasaka, H Terayama
Jan 1, 1982·Methods in Enzymology·J Finne, T Krusius
Jan 18, 1980·Biochimica Et Biophysica Acta·L Svennerholm, P Fredman
Oct 1, 1993·Journal of Cellular Biochemistry·E Papakonstantinou, G N Misevic

❮ Previous
Next ❯

Citations

Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·E PapakonstantinouL H Block
Sep 16, 2004·Development, Growth & Differentiation·Tetsuya Kominami, Hiromi Takata
Oct 29, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·E PapakonstantinouM Roth
Oct 8, 1999·Archives of Biochemistry and Biophysics·E PapakonstantinouT Sklaviadis
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gradimir MisevicOctavian Popescu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.