A novel class of embryonic cell adhesion glycan epitopes is expressed in human colon carcinomas

Journal of Molecular Recognition : JMR
G N Misevic, O Popescu

Abstract

A new class of acidic glycans isolated from marine sponges and sea urchin embryos was shown to mediate cell adhesion via carbohydrate-carbohydrate interactions. Cell aggregation blocking monoclonal antibodies (Block 1 and Block 2 mAbs) directed against these polysaccharides localized functional epitopes in embryonic sea urchin gut. Immunofluorescence light microscopy of human colon carcinomas and healthy colon samples with Block 1 and Block 2 mAbs established that the carbohydrate structures similar to the invertebrate acidic glycan adhesion molecules are also expressed in humans. The Block 1 mAb labeled basal and apical lamina of tumor cells, whereas the Block 2 bound exclusively to the apical part of the epithelium. In normal tissue whole goblet cell membrane was stained with both antibodies indicating that transformation leads to spatial rearrangement of glycan antigens. Acidic glycans from human colon carcinomas and normal colon were isolated after delipidation, and complete pronase and DNase digestion, by gel filtration and adsorption to anion exchange membranes. Immunodot assay with Block 1 and Block 2 mAbs revealed that tumor cells have elevated expression of both carbohydrate structures. These results suggest that the a...Continue Reading

Citations

Jan 1, 2004·The Journal of Biological Chemistry·Gradimir N MisevicGerard Strecker
Mar 31, 1999·Microscopy Research and Technique·X Fernàndez-Busquets, M M Burger
Jul 23, 2014·Asian-Australasian Journal of Animal Sciences·Xinhong LuanShuwei Zhang
Jan 17, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gradimir Misevic, Emanuela Garbarino
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gradimir MisevicOctavian Popescu

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