A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nanogold colloids assembled on a polytyramine-modified gold electrode

Analytica Chimica Acta
Mahmoud LabibBo Mattiasson

Abstract

A highly sensitive competitive capacitive glucose biosensor was constructed based on gold nanoparticles, which were employed as a platform to immobilize concanavalin A (Con A). Gold nanoparticles were fixed on a gold electrode, on which a layer of polytyramine was preformed via electrochemical polymerization. The sensing mechanism is based on the competitive dissociation of a glucose polymer or a glycoconjugate from the glycoligand binding sites of immobilized Con A by the added glucose. To further improve the sensor response, several glucose polymers as well as a synthesized glycoconjugate using the periodate method, were screened. Consequently, dextran (MW 39 kDa) was selected and the feasibility of the proposed biosensor was evaluated for a competitive assay of glucose. Experimental results show that the biosensor responded linearly to glucose in the range from 1.0 x 10(-6) to 1.0 x 10(-2) M, corresponding to 0.18 microg mL(-1) to 1.8 mg mL(-1) of glucose with a detection limit of 1.0 x 10(-6) M under optimized conditions. The studied biosensor exhibited a response time of about 15 min and a neglectable loss in sensitivity after 10 repeated analytical cycles.

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Citations

Sep 10, 2011·Chemical Reviews·Qian WuZhefu Chen
Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Jul 20, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sanja TrajkovicYuguang Cai
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Feb 16, 2013·Enzyme and Microbial Technology·Juanjuan ZhangXia Zhong
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Jul 16, 2013·Chemical Society Reviews·Jeffrey KirschAleksandr Simonian
Jan 9, 2013·Journal of Diabetes Science and Technology·Xian HuangQiao Lin
Feb 22, 2017·Sensors·Gizem Ertürk, Bo Mattiasson
Aug 21, 2013·Journal of Micromechanics and Microengineering : Structures, Devices, and Systems·Xian HuangQiao Lin
Dec 9, 2015·Materials·Baozhen Wang, Jun-Ichi Anzai
Oct 30, 2018·Applied Biochemistry and Biotechnology·Gizem Ertürk BergdahlBo Mattiasson
Apr 26, 2019·Applied Biochemistry and Biotechnology·Gizem Ertürk BergdahlBo Mattiasson
Feb 14, 2012·Analytical Chemistry·Mahmoud LabibMaxim V Berezovski

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