A novel composite immunotoxin that suppresses rabies virus production by the infected cells.

Journal of Immunological Methods
Tatiana MareevaYuri Sykulev

Abstract

Using strepavidin as a scaffold, we have assembled a composite immunotoxin that consists of recombinant Pseudomonas exotoxin A subunit (PE38) and recombinant 25-D1.16 Fab fragment which recognizes the SIINFEKL (pOV8) peptide from ovalbumin in association with H-2K(b) MHC class I protein. The composite immunotoxin exercises cytotoxicity against H-2K(b+) cells sensitized with pOV8 peptide but not with irrelevant peptide. Specific binding of the immunotoxin to H-2K(b+) cells infected with recombinant rabies virus (RV) expressing pOV8 epitope (RV-pOV8) resulted in the suppression of the production of virus particles by the infected cells. This strategy allows readily produce different immunotoxins with desired specificity by combining various targeting and toxin molecules. The results provide a proof of concept that composite immunotoxins can be utilized as novel immunotherapeutics to stop virus spread in the acute phase of the infection allowing winning time for the development of protective immune response.

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Citations

Mar 29, 2014·PloS One·Nicholas O FischerCraig D Blanchette
Jul 10, 2012·International Immunopharmacology·Hong-Ru LiangXian-Zhu Xia
Apr 17, 2014·International Immunopharmacology·Hong-Ru LiangXian-Zhu Xia
Nov 12, 2014·Antimicrobial Agents and Chemotherapy·Eileen M GeogheganRichard B Markham
Jul 14, 2016·ACS Applied Materials & Interfaces·Sean F GilmorePaul T Henderson
Jan 6, 2016·Journal of Leukocyte Biology·Katja SpiessMette M Rosenkilde
Jul 24, 2020·Bioconjugate Chemistry·Martine DarwishCraig Blanchette

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