A novel contractile phenotype with cardiac transgenic expression of the human P2X4 receptor

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Bing HuB T Liang

Abstract

The P2X4 receptor is a newly identified receptor expressed in the heart cell. Its function was elucidated with cardiac transgenic (TG) expression of the receptor by using the myocardium-specific a-myosin heavy chain promoter. The presence of the transgene was determined by polymerase chain reaction by using primers specific to the receptor and the vector linker region, by Southern blotting of the genomic DNA, and by immunoblotting and immunohistochemistry of both isolated cardiac myocytes and intact hearts. In intact heart study, the P2X4 receptor TG mouse exhibited significantly elevated basal cardiac contractility with greater rates of contraction and relaxation, left ventricular developed pressure, and cardiac output compared with nontransgenic (NTG) animals but showed no evidence of hypertrophy or heart failure. The TG heart also showed a greater increase of cardiac contractility in response to the P2X receptor agonist 2-methylthioATP, consistent with overexpression of a functional P2X4 receptor with consequent increase in the receptor-mediated response. In isolated cardiac cell study, the TG heart cell showed a similar level of basal contraction amplitude as the NTG heart cell while exhibiting a threefold greater increase ...Continue Reading

References

Jun 1, 1977·The Journal of Physiology·T Forrester, C A Williams
Jul 1, 1991·Circulation·V Ralevic, G Burnstock
Oct 1, 1989·The American Journal of Physiology·M B De Young, A Scarpa
Nov 1, 1982·Acta Physiologica Scandinavica·B B FredholmM Wennmalm
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·F SotoW Stühmer
Jun 14, 1996·Biochemical and Biophysical Research Communications·F SotoW Stühmer
Nov 15, 1996·The Journal of Clinical Investigation·C E MurryS M Schwartz
Mar 1, 1997·Trends in Pharmacological Sciences·B B FredholmM Williams
Dec 5, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C DoughertyB T Liang
Feb 5, 1999·Brain Research. Molecular Brain Research·A Townsend-NicholsonG Burnstock
Mar 29, 2001·Physiological Reviews·G Vassort
Jun 14, 2001·Diabetes Research and Clinical Practice·W GatlingUNKNOWN Poole Diabetes Study
Jun 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Q Mei, B T Liang

❮ Previous
Next ❯

Citations

May 2, 2012·Purinergic Signalling·Karina Kaczmarek-HájekAnnette Nicke
May 17, 2005·Journal of Molecular and Cellular Cardiology·Johanna BaloghDavid Erlinge
Jan 14, 2004·Molecular Biology of the Cell·Guangwei DuMichael A Frohman
Jun 22, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·David C MontroseDaniel W Rosenberg
Mar 5, 2010·The Journal of Pharmacology and Experimental Therapeutics·Si-Yuan ZhouBruce T Liang
Jul 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Dmitry SoninBruce T Liang
Feb 10, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Jian-Bing ShenBruce T Liang
Mar 8, 2012·Circulation Research·Stephanie GuoPaul S Brookes
Mar 8, 2011·Circulation Journal : Official Journal of the Japanese Circulation Society·Yuichiro OhataToshiaki Kawai
Jun 8, 2011·International Heart Journal·Paul FacerPraveen Anand
Mar 12, 2013·Journal of Cardiovascular Pharmacology·Siyuan ZhouBruce T Liang
Feb 24, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Hanny MusaHalina Dobrzynski
Jun 22, 2010·Histochemistry and Cell Biology·K BarthM Kasper
Mar 10, 2015·Computational and Structural Biotechnology Journal·Ronghua YangBruce T Liang
Feb 28, 2002·The Journal of Biological Chemistry·Bing HuBruce T Liang
Aug 4, 2012·Circulation Research·Ronghua Yang, Bruce T Liang
Mar 14, 2014·Circulation. Heart Failure·Tiehong YangBruce T Liang
Oct 15, 2008·Hypertension·Julian Palomino-DozaBernard Keavney
Feb 25, 2006·Acta Physiologica·K B AnderssonG Christensen
Oct 17, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jian-Bing ShenBruce T Liang
Sep 18, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Jian-Bing ShenBruce T Liang
Feb 12, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Ulrich GergsJoachim Neumann
Sep 23, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Jian-Bing ShenBruce T Liang
Feb 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jian-Bing ShenBruce T Liang
Feb 5, 2003·The Journal of Biological Chemistry·Akos ZsemberyErik M Schwiebert
Apr 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dorianna SandonàRomeo Betto
Dec 21, 2014·Purinergic Signalling·Geoffrey Burnstock, Amir Pelleg
Jul 19, 2020·International Journal of Molecular Sciences·Bruno Bragança, Paulo Correia-de-Sá
Dec 17, 2014·Purinergic Signalling·Kwok-Kuen CheungGeoffrey Burnstock
May 8, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Alexander YangBruce T Liang
Oct 31, 2020·British Journal of Pharmacology·Peter IllesFrancesco Di Virgilio
Jan 2, 2021·International Journal of Molecular Sciences·Sun-Hee Woo, Tran Nguyet Trinh

❮ Previous
Next ❯

Related Concepts

Related Papers

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Jian-bing ShenBruce T Liang
American Journal of Physiology. Heart and Circulatory Physiology
Dmitry SoninBruce T Liang
American Journal of Physiology. Heart and Circulatory Physiology
Jian-Bing ShenBruce T Liang
© 2021 Meta ULC. All rights reserved