A novel copper A containing menaquinol NO reductase from Bacillus azotoformans

Biochemistry
SuhartiS de Vries

Abstract

The molecular biology and biochemistry of denitrification in gram-negative bacteria has been studied extensively. However, little is known about this process in gram-positive bacteria. We have purified the NO reductase from the cytoplasmic membrane of the gram-positive bacterium Bacillus azotoformans. The purified enzyme consists of two subunits with apparent molecular masses of 16 and 40 kDa based on SDS-PAGE. Analytical and spectroscopic determinations revealed the presence of one non-heme iron, two copper atoms and of two b-type hemes per enzyme complex. Heme c was absent. Using EPR and UV-visible spectroscopy, it was determined that one of the hemes is a low-spin heme b, in which the two axial histidine imidazole planes are positioned at an angle of 60-70 degrees. The second heme b is high-spin binding CO in the reduced state. The high-spin heme center and the non-heme iron are EPR silent. They are proposed to form a binuclear center where reduction of NO occurs. There are two novel features of this enzyme that distinguish it from other NO reductases. First, the enzyme contains copper in form of copper A, an electron carrier up to now only detected in cytochrome oxidases and nitrous oxide reductases. Second, the enzyme uses...Continue Reading

References

Feb 15, 1987·Analytical Biochemistry·E A Berry, B L Trumpower
Nov 1, 1985·Journal of Bacteriology·R Meganathan, R Coffell
Mar 20, 1995·Analytical Biochemistry·A J Brenner, E D Harris
Mar 14, 1994·FEBS Letters·M Saraste, J Castresana
Nov 14, 1997·Journal of Bacteriology·R CrammB Friedrich

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Citations

Jun 29, 2007·Applied Microbiology and Biotechnology·Shi-Han ZhangYao Shi
Oct 3, 2002·Biochimica Et Biophysica Acta·Laurent Philippot
Mar 28, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stephen Spiro
Oct 6, 2005·Applied and Environmental Microbiology·Rajkumari KumaraswamyGerard Muyzer
Jul 25, 2006·Bioscience, Biotechnology, and Biochemistry·Miho SuzukiYasuo Igarashi
Nov 24, 2004·Biological Chemistry·Oliver Einsle, Peter M H Kroneck
Jul 8, 2015·FEBS Letters·Sinan Al-Attar, Simon de Vries
May 3, 2011·Systematic and Applied Microbiology·Ines VerbaendertKim Heylen
Jan 12, 2011·Journal of Biotechnology·Beate KraftHalina E Tegetmeyer
Jan 27, 2011·Biochemical Society Transactions·Ines VerbaendertKim Heylen
Jul 18, 2015·Inorganic Chemistry·Tatsuya SuzukiKazunari Yoshizawa
Oct 18, 2011·Biochimica Et Biophysica Acta·Tomoya HinoYoshitsugu Shiro
Apr 25, 2008·Methods in Enzymology·Sarah J FieldNicholas J Watmough
Oct 18, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Dwaipayan Dutta GuptaShyamalava Mazumdar
Feb 26, 2016·Environmental Microbiology·Constance A RocoJames P Shapleigh
Jun 28, 2014·Journal of the Royal Society, Interface·Anne-Lise DucluzeauWolfgang Nitschke
Dec 8, 2019·Biological Chemistry·Constance PorriniSeav-Ly Tran
Mar 20, 2002·The Journal of Biological Chemistry·Sarah J FieldDavid J Richardson
Jul 21, 2017·Antonie van Leeuwenhoek·Yihua SunAnne Willems
Sep 12, 2007·Journal of the American Chemical Society·Gabriela N LedesmaAlejandro J Vila
Nov 26, 2004·Journal of the American Chemical Society·Shen LuPierre Moënne-Loccoz

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