A novel copper (II) complex activated both extrinsic and intrinsic apoptotic pathways in liver cancerous cells

Journal of Cellular Biochemistry
Azadeh RezaeiSoudeh Khanamani Falahati-Pour

Abstract

Recent advances have put fundamental focus on the application of copper (II) (Cu [II]) complexes as agents for fighting against cancer. To determine whether [Cu(L)(2imi)] complex as a novel Cu complex can induce apoptosis in HepG2 as cancerous cells and L929 as normal cells via extrinsic or intrinsic apoptotic pathways, both cell lines were treated for 24 and 48 hours at IC50 concentrations of [Cu(L)(2imi)] complex. Then, the expression of some apoptosis-related genes including p53, caspase-8, bcl-2, and bax were assayed by real-time polymerase chain reaction. The [Cu(L)(2imi)] complex seems to inhibit the expression of bcl-2 in complex-treated HepG2 cancerous cells following the 24- and 48-hour treatment. The complex upregulated the p53, bax, and caspase-8 genes, therefore treatment of HepG2 cancerous cells with [Cu(L)(2imi)] complex induces programmed cell death via the upregulation of relative bax/bcl-2 ratio. Finally, this copper complex triggered apoptosis in HepG2 cells via both intrinsic and extrinsic pathway, whereas treatment of normal L929 cells with this complex induce apoptosis only via intrinsic pathway with the upregulation of relative bax/bcl-2 ratio and does not affect the expression level of caspase-8 gene and ...Continue Reading

References

Jan 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R J StockertI Sternlieb
Apr 26, 1969·Nature·B RosenbergV H Mansour
Feb 1, 1984·Journal of Medicinal Chemistry·A W TaiT A Khwaja
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Aug 28, 1998·Science·D R Green, J C Reed
Nov 5, 1999·Advances in Cancer Research·G J Kelloff
Jun 27, 2001·Trends in Molecular Medicine·J C Reed
Nov 21, 2001·Nature Cell Biology·K F Ferri, G Kroemer
Oct 3, 2003·Ageing Research Reviews·Jian-Hua ZhangBrian Herman
Nov 26, 2003·Cellular Signalling·Andrew Thorburn
Feb 24, 2005·Cancer Biology & Therapy·Zhaoyu Jin, Wafik S El-Deiry
Nov 5, 2005·Acta Biochimica Et Biophysica Sinica·Ting-Jun FanJin Liang
Feb 16, 2006·Anti-cancer Agents in Medicinal Chemistry·Irena Kostova
Apr 3, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Masaki KawakamiMasaaki Kurasaki
Sep 2, 2008·The Journal of Biological Chemistry·Michelle L Turski, Dennis J Thiele
Feb 10, 2009·Anti-cancer Agents in Medicinal Chemistry·Cristina MarzanoCarlo Santini
Apr 10, 2009·Current Medicinal Chemistry·Saverio Tardito, Luciano Marchiò
Jul 25, 2009·Medicinal Research Reviews·Francesco TisatoCarlo Santini
Sep 18, 2010·Current Medicinal Chemistry·L Ruiz-Azuara, M E Bravo-Gómez
Aug 31, 2012·Mini Reviews in Medicinal Chemistry·Zdenek Skrott, Boris Cvek
Feb 15, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S JainL T Vahdat
May 3, 2013·Chemical Communications : Chem Comm·Nicolas P E Barry, Peter J Sadler
Oct 10, 2013·Chemical Reviews·Carlo SantiniCristina Marzano
Jul 12, 2014·International Journal of Biological Sciences·Joong Sup Shim, Jun O Liu
Aug 15, 2014·Biological Trace Element Research·Lorena BeccoBeatriz Garat
Feb 13, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mona SalimiHassan Sanati
Apr 23, 2015·European Journal of Medicinal Chemistry·Jinxu QiHong Liang
Oct 26, 2016·Molecular Medicine Reports·Katarína KoňarikováIngrid Žitňanová
Apr 12, 2017·Cytotechnology·Farzaneh Sadat HosseiniMehdi Mahmoodi
May 28, 2017·Life Sciences·Mojgan Noroozi KarimabadGholamhossein Hassanshahi
Feb 13, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Fatemeh MohammadizadehMehdi Mahmoodi
Feb 13, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qingfang HanAihua Li
Sep 27, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Hamid Bakhshi AliabadMehdi Mahmoodi

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