A novel covalent coupling method for coating of capillaries with liposomes in capillary electrophoresis

Electrophoresis
Jie MeiYu-Qi Feng

Abstract

A novel covalent coupling method for coating of capillaries with liposomes has been developed, which includes three steps: (i) epoxy-diol coating, (ii) activation with 2,2,2-trifluoroethanesulfonyl chloride, and (iii) liposome coupling. The coating conditions, such as the reaction time and temperature of liposome coupling, the content of dimyristoylphosphatidylethanolamine in liposomes, were optimized. Vesicles were visualized on the inner silica wall as confirmed by atomic force microscopy. The effectiveness of the coating was demonstrated by investigating the effect of pH of BGE on EOF and separating neutral compounds. The intra- and inter-capillary variations in EOF are 4.02% RSD (n=30) and 6.72% RSD (n=4) respectively, and the coated capillaries can be used to perform analysis at least for one month without any performance deterioration when stored at 4 degrees C. A set of drugs with diverse structures was applied into the developed liposome-coated CE. The normalized capacity factor (K) was introduced to quantitatively evaluate drug-membrane interactions. The relationship between log K and the fraction dose absorbed in humans (Fa%) shows that the liposome-coated CE can be utilized for in vitro prediction of Fa% of drugs tha...Continue Reading

References

Mar 7, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·T OsterbergP Lundahl
Feb 28, 2002·Analytical Chemistry·Jennifer M CunliffeCharles A Lucy
Apr 11, 2003·Journal of Chromatography. a·Giulia ManettoZdenek Deyl
Aug 22, 2003·Journal of Chromatography. a·Jari T HautalaMarja-Liisa Riekkola
Jul 23, 2004·Journal of Medicinal Chemistry·Stefan WillmannJennifer B Dressman
Nov 10, 2004·Journal of Chromatography. a·Maria V LindénMarja-Liisa Riekkola
Apr 13, 2005·Electrophoresis·Susanne K WiedmerMarja-Liisa Riekkola
Jul 15, 2005·Journal of Chromatography. a·Sami J O VarjoMarja-Liisa Riekkola

❮ Previous
Next ❯

Citations

Nov 3, 2009·Journal of Separation Science·Juan QiaoHuimin Ma
Jun 12, 2009·Electrophoresis·Susanne K Wiedmer, Ruth Shimmo
Feb 18, 2010·Electrophoresis·Stefan BachmannGünther K Bonn
Mar 10, 2016·Electrophoresis·Shane S WellsChristopher R Harrison
Nov 16, 2010·Biomedical Chromatography : BMC·T Cserháti, M Szögyi
Jul 14, 2017·Electrophoresis·Vojtech Adam, Marketa Vaculovicova
Aug 18, 2020·Critical Reviews in Analytical Chemistry·Guangyan LiuJianping Li
May 26, 2010·Analytical Chemistry·Nicholas W FrostMichael T Bowser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.