A novel CPx-ATPase from the cadmium hyperaccumulator Thlaspi caerulescens

FEBS Letters
Catherine BernardNathalie Verbruggen

Abstract

Thlaspi caerulescens exhibits a unique capacity for cadmium tolerance and accumulation. We investigated the molecular basis of this exceptional Cd(2+) tolerance by screening for T. caerulescens genes, which alleviate Cd(2+) toxicity upon expression in Saccharomyces cerevisiae. This allowed for the isolation of a cDNA encoding a peptide with homology to the C-terminal part of a heavy metal ATPase. The corresponding TcHMA4 full-length sequence was isolated from T. caerulescens and compared to its homolog from Arabidopsis thaliana (AtHMA4). Expression of TcHMA4 and AtHMA4 cDNAs conferred Cd sensitivity in yeast, while expression of TcHMA4-C and AtHMA4-C cDNAs encoding the C-termini of, respectively, TcHMA4 and AtHMA4 conferred Cd tolerance. Moreover, heterologous expression in yeast suggested a higher Cd binding capacity of TcHMA4-C compared to AtHMA4-C. In planta, both HMA4 genes were expressed at a higher level in roots than in shoots. However, TcHMA4 shows a much higher constitutive expression than AtHMA4. Our data indicate that HMA4 could be involved in Cd(2+) transport and possibly in the Cd hyperaccumulation character.

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G NuciforaS Silver
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 1, 1996·Trends in Biochemical Sciences·M Solioz, C Vulpe
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·C RensingB P Rosen
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·S ClemensJ I Schroeder
Sep 28, 1999·Journal of Bacteriology·C RensingB P Rosen
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·C RensingB P Rosen
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z WeissmanD Kornitzer
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·N S PenceL V Kochian
Jun 13, 2001·Plant Physiology·K B Axelsen, M G Palmgren
Feb 16, 2002·Journal of Experimental Botany·Fang-Jie ZhaoSteve P McGrath
Sep 12, 2002·Annual Review of Plant Biology·Christopher Cobbett, Peter Goldsbrough
Apr 23, 2003·Current Genetics·Anna L Okorokova-FaçanhaKarl Ekwall
Apr 26, 2003·Trends in Biochemical Sciences·Thijs J G EttemaJohn van der Oost
Jul 10, 2003·The Plant Journal : for Cell and Molecular Biology·Rebecca F MillsLorraine E Williams
Apr 22, 2004·The Plant Cell·Dawar HussainChristopher S Cobbett

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Citations

Dec 8, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Joanna Deckert
Jan 3, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Robert A IngleLee J Sweetlove
Dec 11, 2008·Environmental Science and Pollution Research International·Abdul R Memon, Peter Schröder
Mar 17, 2010·Biological Trace Element Research·Lingyan HouHong Shen
Apr 16, 2005·Current Opinion in Biotechnology·Ute Krämer
Mar 6, 2009·International Journal of Phytoremediation·Shuhe Wei, Qixing Zhou
Dec 4, 2010·Journal of Experimental Botany·Daisei UenoJian Feng Ma
Apr 21, 2011·Journal of Experimental Botany·Joshua P AdamsCetin Yuceer
Oct 27, 2010·PloS One·Rebecca F MillsLorraine E Williams
Jan 15, 2014·Journal of Experimental Botany·Oskar SiemianowskiDanuta Maria Antosiewicz
Mar 3, 2010·Annual Review of Plant Biology·Ute Krämer
Apr 28, 2007·FEBS Letters·Ute KrämerMarc Hanikenne
Nov 26, 2015·International Journal of Molecular Sciences·Songqing TianSuzhen Huang
May 3, 2011·Current Opinion in Plant Biology·Marc Hanikenne, Cécile Nouet
Sep 15, 2012·The Plant Journal : for Cell and Molecular Biology·Thomas SchneiderStefan Meyer
Sep 11, 2007·Biochemical and Biophysical Research Communications·Aravind ParameswaranHendrik Küpper
Nov 20, 2008·Journal of Integrative Plant Biology·Giovanni DalCorsoAntonella Furini
Feb 9, 2007·The New Phytologist·Martin R BroadleyAlexander Lux
Dec 5, 2008·The New Phytologist·Ronald J F J OomenSébastien Thomine
Nov 15, 2007·Plant, Cell & Environment·Wilfried H O ErnstDirk Wesenberg
Feb 5, 2009·The New Phytologist·Nathalie VerbruggenHenk Schat
Nov 23, 2012·Plant, Cell & Environment·Oskar SiemianowskiDanuta Maria Antosiewicz

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