A novel cytochrome P450 CYP6AB14 gene in Spodoptera litura (Lepidoptera: Noctuidae) and its potential role in plant allelochemical detoxification

Journal of Insect Physiology
Rui-Long WangRen Sen Zeng

Abstract

Cytochrome P450 monooxygenases (P450s) play a prominent role in the adaptation of insects to host plant chemical defenses. To investigate the potential role of P450s in adaptation of the lepidopteran pest Spodoptera litura to host plant allelochemicals, an expressed sequence data set derived from 6th instar midgut tissues was first mined. One sequence identified from the S. litura 6th instar midgut EST database was determined by phylogenetic analysis to belong to the CYP6AB P450 subfamily, and named CYP6AB14. Dietary supplementation of S. litura larvae with either xanthotoxin (XAN), coumarin (COU) and flavone (FLA) led to elevated CYP6AB14 transcript levels in both midgut and fat body tissues. Injection of CYP6AB14-derived double-stranded RNA (dsRNA) into S. litura individuals significantly reduced CYP6AB14 transcript levels, and resulted in increased developmental abnormalities and higher mortality rates among XAN, COU and FLA-fed larvae. Our results strongly suggest a key role for CYP6AB14 in plant allelochemical detoxification in S. litura.

References

Oct 1, 1998·Insect Biochemistry and Molecular Biology·N Liu, J G Scott
Nov 11, 1998·Archives of Biochemistry and Biophysics·V M GuzovR Feyereisen
Nov 7, 2001·Pest Management Science·J G Scott, Z Wen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 16, 2002·European Journal of Biochemistry·Teresa LuqueDavid R O'Reilly
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xianchun LiMary A Schuler
Mar 20, 2004·European Journal of Biochemistry·Marcel AmichotJean-Baptiste Bergé
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Aug 24, 2006·Annual Review of Entomology·Xianchun LiMay R Berenbaum
Feb 28, 2007·Trends in Ecology & Evolution·Laurence DesprésChristiane Gallet
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 6, 2007·Nature Biotechnology·James A BaumJames Roberts
Jul 6, 2010·Biochimica Et Biophysica Acta·R Feyereisen
Aug 26, 2010·Biochimica Et Biophysica Acta·David R Nelson
Oct 5, 2010·Biochimica Et Biophysica Acta·Mary A Schuler
Jan 12, 2011·Insect Biochemistry and Molecular Biology·Guodong NiuMay R Berenbaum
Aug 29, 2012·Molecular Ecology·Mary A Schuler
Jan 9, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David R NelsonJohn J Stegeman
Apr 18, 2013·Bulletin of Entomological Research·X ZhangJ Zhao
Oct 5, 2013·Journal of Proteome Research·Vishal V DawkarAshok P Giri
Oct 1, 1985·Journal of Chemical Ecology·M Berenbaum, J J Neal
Apr 25, 2014·Pesticide Biochemistry and Physiology·Adeel Rehan, Shoaib Freed

❮ Previous
Next ❯

Citations

Feb 24, 2016·Frontiers in Physiology·Vijaya Sudhakara Rao KolaMaganti S Madhav
May 10, 2019·International Journal of Molecular Sciences·Muhammad HafeezMo Wang
Dec 30, 2020·Current Opinion in Insect Science·Marilou VandenholeThomas Van Leeuwen
Jan 2, 2021·Current Opinion in Insect Science·Xianchun LiXuewei Chen
Jan 17, 2021·International Journal of Biological Macromolecules·Yuan-Chen ZhangKun-Peng Zhang
Mar 28, 2021·Pesticide Biochemistry and Physiology·Li ShiXiaolan Liao
Aug 21, 2021·Functional & Integrative Genomics·Archana SinghIndrakant Kumar Singh
Sep 2, 2021·Insect Biochemistry and Molecular Biology·Dries AmezianGaëlle Le Goff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.