A novel diagnostic approach to detecting porcine epidemic diarrhea virus: The lateral immunochromatography assay

Journal of Virological Methods
Yong Kwan KimDong-Jun An

Abstract

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea and dehydration in sucking piglets and has a high mortality rate. An immunochromatography (IC) assay, known as a lateral flow test, is a simple device intended to detect the presence of target pathogens. Here, we developed an IC assay that detected PEDV antigens with 96.0% (218/227) sensitivity and 98.5% (262/266) specificity when compared with real-time reverse transcriptase (RT)-PCR using FAM-labeled probes based on sequences from nucleocapsid genes. The detection limits of the real-time RT-PCR and IC assays were 1×10(2) and 1×10(3) copies, respectively. The IC assay developed herein did not detect non-specific reactions with other viral or bacterial pathogens, and the assay could be stored at 4°C or room temperature for 15 months without affecting its efficacy. Thus, the IC assay may result in improved PED detection and control on farms, and is a viable alternative to current diagnostic tools for PEDV.

References

Jan 1, 1978·Archives of Virology·M B Pensaert, P de Bouck
Apr 20, 1996·The Veterinary Record·K de Verdier Klingenberg, J Esfandiari
Feb 1, 1996·Acta Chemica Scandinavica·M P Laitinen, M Vuento
Jul 3, 1999·The Veterinary Record·G C PritchardG Ibata
Jul 6, 2000·Scandinavian Journal of Infectious Diseases·B P BerdalH Meyer
Jun 12, 2001·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J BuserJ Munzinger
Apr 11, 2006·Clinical and Vaccine Immunology : CVI·Jin-Sik OhDae-Sub Song
Jun 23, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Dae S SongBong K Park
Mar 11, 2008·The Veterinary Record·P MartelliM B Pensaert
Sep 16, 2008·Analytical and Bioanalytical Chemistry·Geertruida A Posthuma-TrumpieAart van Amerongen
Jul 25, 2009·Emerging Infectious Diseases·Suphasawatt PuranavejaRoongroje Thanawongnuwech
Jun 15, 2010·Archives of Virology·Jianfei ChenLi Feng
Aug 25, 2011·Analytical and Bioanalytical Chemistry·Shruti ShuklaMyunghee Kim
Aug 15, 2012·Analytical Chemistry·Gina ZhouDavid Juncker
Sep 11, 2012·International Journal of Molecular Medicine·Tingxiu XiangGuoqi Lai

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Citations

May 9, 2019·International Journal of Molecular Sciences·I-Li LiuHui-Wen Chen
Jul 2, 2016·Essays in Biochemistry·Katarzyna M Koczula, Andrea Gallotta
Aug 16, 2021·Journal of Virological Methods·Xiaotong YangYouxiang Diao

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
antibody sandwich assay

Software Mentioned

Statistical Package for the Social Sciences ( SPSS )

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