A Novel Dicyanoisophorone-Based Ratiometric Fluorescent Probe for Selective Detection of Cysteine and Its Bioimaging Application in Living Cells

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Hengrui ZhangZhijie Fang

Abstract

A selective and ratiometric turn-on fluorescent probe was designed and synthesized by using a novel dicyanoisophorone-based derivative and acrylate moiety. The probe displayed high stability and good selectivity to cysteine (Cys) over homocysteine (Hcy) and glutathione (GSH). It also exhibited rapid response to Cys within 180 s. Most importantly, the fluorescence intensity ratio at 590 and 525 nm (I590/I525) was linearly dependent on the Cys concentration in the range from 0 to 40 μM and the detection limit calculated to be 0.48 μM. This probe was also applied for bioimaging of intracellular Cys in living HeLa cells with low cytotoxicity.

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Citations

Jul 14, 2019·Analytical and Bioanalytical Chemistry·Sujoy DasPrithidipa Sahoo
Sep 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Song ChenLei Liu
Jan 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaohua MaLin Liu
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan ZhengSong Chen

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Methods Mentioned

BETA
Fluorescence
column chromatography
Fluorescence Spectroscopy

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