A novel DNA methylation panel accurately detects colorectal cancer independently of molecular pathway

Journal of Translational Medicine
Micaela FreitasCarmen Jerónimo

Abstract

Colorectal cancer (CRC) is one of the most incident cancers, associated with significant morbidity and mortality, and usually classified into three main molecular pathways: chromosomal instability, microsatellite instability (MSI) and CpG island methylator phenotype (CIMP). Currently, available screening methods are either costly or of limited specificity, impairing global implementation. More cost-effective strategies, including DNA methylation-based tests, might prove advantageous. Although some are already available, its performance is suboptimal, entailing the need for better candidate biomarkers. Herein, we tested whether combined use of APC, IGF2, MGMT, RASSF1A, and SEPT9 promoter methylation might accurately detect CRC irrespective of molecular subtype. Selected genes were validated using formalin-fixed paraffin-embedded tissues from 214 CRC and 50 non-malignant colorectal mucosae (CRN). Promoter methylation levels were assessed using real-time quantitative methylation-specific PCR. MSI and CIMP status were determined. Molecular data were correlated with standard clinicopathological features. Diagnostic and prognostic performances were evaluated by receiver operator characteristics curve and survival analyses, respective...Continue Reading

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Citations

Jul 10, 2019·Alimentary Pharmacology & Therapeutics·Ella MozdiakRamesh P Arasaradnam
Jul 22, 2019·Cancers·Giorgio MalpeliClaudio Bassi
Oct 19, 2019·International Journal of Molecular Sciences·Aldona Kasprzak, Agnieszka Adamek
Jan 29, 2020·World Journal of Gastroenterology : WJG·Chih-Hsiung HsuYu-Ching Chou
Oct 19, 2019·World Journal of Clinical Cases·Yu-Xia Zhan, Guang-Hua Luo
Jan 3, 2021·Cancer Genetics·Khadijeh JamialahmadiMohammad Amin Kerachian
Jun 22, 2021·Frontiers in Cell and Developmental Biology·Omayma MazoujiHicham Mansour
Jul 3, 2021·Oncology Reports·Can Kong, Tao Fu
Sep 2, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Raju KandimallaAjay Goel

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Methods Mentioned

BETA
surgical resection
PCR
biopsies

Software Mentioned

GraphPad Prism
SPSS Statistics

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