A novel DNA polymerase homologous to Escherichia coli DNA polymerase I from a higher plant, rice (Oryza sativa L.)

Nucleic Acids Research
S KimuraK Sakaguchi

Abstract

A novel DNA polymerase, designated as OsPolI-like, has been identified from the higher plant, rice (Oryza sativa L. cv. Nipponbare). The OsPolI-like cDNA was 3765 bp in length, and the open reading frame encoded a predicted product of 977 amino acid residues with a molecular weight of 100 kDa. The OsPolI-like gene has been mapped to chromosome 8 and contains 12 exons and 11 introns. The encoded protein showed a high degree of sequence and structural homology to Escherichia coli pol I protein, but differed from DNA polymerase gamma and theta. The DNA polymerase domain of OsPolI-like showed DNA polymerase activity. Subcellular fractionation analysis suggested that the protein is localized in the plastid. Northern and western blotting, and in situ hybridization analyses demonstrated preferential expression of OsPolI-like in meristematic tissues such as shoot apical meristem, root apical meristem, leaf primordia and the marginal meristem. Interestingly, no expression was detected in mature leaves, although they have a high chloroplast content. These properties indicated that OsPolI-like is a novel plant DNA polymerase. The function of OsPolI-like is discussed in relation to plastid maturation.

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Citations

May 11, 2005·Journal of Molecular Evolution·Junko Kusumi, Hidenori Tachida
Apr 5, 2005·Journal of Plant Research·Taichi YamamotoKengo Sakaguchi
May 25, 2007·Plant Molecular Biology·Ryo TakeuchiKengo Sakaguchi
Jul 5, 2005·Biochemical and Biophysical Research Communications·Yoko MoriKengo Sakaguchi
Jun 1, 2005·Molecular and Biochemical Parasitology·Fiona SeowMichael P Barrett
Apr 23, 2003·Biochemical and Biophysical Research Communications·Yoshiyuki MizushinaKengo Sakaguchi
May 23, 2008·Critical Reviews in Plant Sciences·Miguel Garcia-Diaz, Katarzyna Bebenek
May 24, 2005·Plant Physiology·Shinya TakahashiAtsushi Tanaka
Mar 24, 2011·Plant Physiology·Jean-Sébastien ParentNormand Brisson
Jun 23, 2004·European Journal of Biochemistry·Yukinobu UchiyamaKengo Sakaguchi
Feb 13, 2016·Frontiers in Plant Science·Stewart A Morley, Brent L Nielsen
Oct 29, 2011·Biochemical and Biophysical Research Communications·Hirofumi KoderaKengo Sakaguchi
Jan 22, 2009·The FEBS Journal·Kengo SakaguchiKazuki Iwabata
Jun 14, 2005·Biochemical and Biophysical Research Communications·Yoshiyuki MizushinaHiromi Yoshida
Mar 2, 2005·Biochemical and Biophysical Research Communications·Seisuke KimuraKengo Sakaguchi
May 30, 2006·Biochemical and Biophysical Research Communications·Ai SaotomeKengo Sakaguchi
Sep 15, 2005·Trends in Microbiology·Jonathan Filée, Patrick Forterre
Nov 6, 2003·Biochemical Pharmacology·Yoshiyuki MizushinaKengo Sakaguchi
Aug 2, 2002·Molecular Cell·Michele M KlingbeilPaul T Englund
Sep 23, 2014·Frontiers in Plant Science·Karin KrupinskaGötz Hensel
Oct 4, 2014·Frontiers in Plant Science·Takashi Moriyama, Naoki Sato
Oct 5, 2017·Nucleic Acids Research·Noe Baruch-Torres, Luis G Brieba
Nov 19, 2015·Frontiers in Plant Science·Delene J Oldenburg, Arnold J Bendich
Jan 18, 2020·International Journal of Molecular Sciences·Nicolas ChevignyJosé Manuel Gualberto
Oct 30, 2004·Genes & Genetic Systems·Tomoya AsanoYasunori Machida
Feb 9, 2006·Chemical Reviews·Seisuke Kimura, Kengo Sakaguchi
May 22, 2003·Biochimica Et Biophysica Acta·Yoshiyuki MizushinaKengo Sakaguchi
Mar 2, 2006·Journal of Agricultural and Food Chemistry·Hiroshi IijimaYoshiyuki Mizushina

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