Abstract
A spontaneous Escherichia coli mutant, named Q3, resistant to nalidixic acid was obtained from a previously described clinical isolate of E. coli, Q2, resistant to fluoroquinolones but susceptible to nalidixic acid (E. Cambau, F. Bordon, E. Collatz, and L. Gutmann, Antimicrob. Agents Chemother. 37:1247-1252, 1993). Q3 harbored the mutation Asp82Gly in addition to the Gly81Asp mutation of Q2. The different mutations leading to Gly81Asp, Asp82Gly, and Gly81AspAsp82Gly were introduced into the gyrA gene harbored on plasmid pJSW102, and the resulting plasmids were introduced into E. coli KNK453 (gyrAts) by transformation. The presence of Asp82Gly or Gly81Asp alone led to a low-level resistance to fluoroquinolones but not to nalidixic acid resistance. When both mutations were present, resistance to both nalidixic acid and fluoroquinolones was expressed. Purified gyrases of the different mutants showed similar rates of supercoiling. Dominance of the various gyrA mutant alleles harbored on plasmids was examined. The susceptibility to quinolones associated with wild-type gyrA was always dominant. The susceptibility to nalidixic acid expressed by the Gly81Asp mutant was dominant, while that expressed by the Asp82Gly mutant was recessive...Continue Reading
References
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M GellertH A Nash
Nov 1, 1979·Journal of Bacteriology·K N Kreuzer, N R Cozzarelli
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L F Liu, J C Wang
Oct 10, 1979·Nucleic Acids Research·A R MorganD E Pulleyblank
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Dec 1, 1992·Antimicrobial Agents and Chemotherapy·S BazileM Essiz
Feb 1, 1991·Antimicrobial Agents and Chemotherapy·P Hallett, A Maxwell
Feb 1, 1991·Antimicrobial Agents and Chemotherapy·M Oram, L M Fisher
Oct 1, 1990·Antimicrobial Agents and Chemotherapy·N J Robillard
Dec 1, 1990·The Journal of Antimicrobial Chemotherapy·G C Crumplin
Mar 1, 1991·Antimicrobial Agents and Chemotherapy·N Moniot-VilleL Gutmann
Jun 1, 1989·Antimicrobial Agents and Chemotherapy·M E CullenL M Fisher
Feb 1, 1989·Antimicrobial Agents and Chemotherapy·D T Chu, P B Fernandes
Jan 1, 1988·Molecular & General Genetics : MGG·H YoshidaS Nakamura
Sep 1, 1986·Molecular & General Genetics : MGG·J YamagishiS Nakamura
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Jul 1, 1969·Journal of Bacteriology·M W Hane, T H Wood
Aug 15, 1970·Nature·U K Laemmli
Jan 1, 1983·Methods in Enzymology·J Messing
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Oct 1, 1994·Antimicrobial Agents and Chemotherapy·J VilaT Jimenez de Anta
Jun 1, 1994·Antimicrobial Agents and Chemotherapy·P Heisig, R Tschorny
Dec 1, 1993·Antimicrobial Agents and Chemotherapy·C J SoussyD C Hooper
Sep 1, 1993·The Journal of Antimicrobial Chemotherapy·A Denis, N J Moreau
Jan 1, 1993·Antimicrobial Agents and Chemotherapy·C J Willmott, A Maxwell
Apr 1, 1993·Antimicrobial Agents and Chemotherapy·P HeisigH Falkenstein-Paul
Apr 1, 1993·Antimicrobial Agents and Chemotherapy·H YoshidaS Nakamura
Jun 1, 1993·Antimicrobial Agents and Chemotherapy·E CambauL Gutmann
Jan 18, 1996·Nature·J M BergerJ C Wang
Mar 1, 1996·Antimicrobial Agents and Chemotherapy·Y KumagaiH Ikeda
Citations
Jun 27, 2001·Applied and Environmental Microbiology·L Miché, J Balandreau
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Xiao-Su PanL Mark Fisher
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·J M LingA F Cheng
Jul 16, 2008·Antimicrobial Agents and Chemotherapy·Michael T BlackChristine Miossec
Dec 1, 2011·Environmental Microbiology Reports·Marcia S OsburneSallie W Chisholm
Feb 6, 2014·PloS One·Vinod ShanmughapriyaM Hussain Munavar
Feb 13, 1999·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·R BachoualC J Soussy
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·X ZhaoK Drlica
Oct 6, 1997·FEMS Microbiology Letters·J M Gómez-GómezJ L Martínez
Oct 20, 2001·Pharmacotherapy·D T Bearden, L H Danziger
Oct 26, 2012·Interdisciplinary Perspectives on Infectious Diseases·Axel Dalhoff
Aug 16, 2019·Clinical Microbiology Reviews·Joaquim Ruiz
Dec 18, 2009·International Journal of Microbiology·M P MontetC Vernozy-Rozand
Oct 26, 2013·Osong Public Health and Research Perspectives·Jun-Young KimSeong-Han Kim
Jul 17, 2014·PloS One·Ishrat J AzmiKaisar A Talukder
Dec 1, 2017·Clinical Microbiology Reviews·Cláudia Gomes, Joaquim Ruiz
Nov 19, 2019·Frontiers in Microbiology·Yiwen YangYinbao Wu
Oct 26, 2018·The Journal of Antimicrobial Chemotherapy·Boas C L van der PuttenConstance Schultsz
Jan 30, 1999·Antimicrobial Agents and Chemotherapy·T TakenouchiM Sugawara
Feb 27, 1999·Antimicrobial Agents and Chemotherapy·D T MoirG F Vovis
Apr 2, 1999·Antimicrobial Agents and Chemotherapy·N A el AminB Wretlind
Sep 28, 2001·Journal of Clinical Microbiology·L E LindlerN Jahan
Jan 7, 1998·Antimicrobial Agents and Chemotherapy·B Waters, J Davies
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Apr 26, 2005·International Journal of Antimicrobial Agents·Katie L HopkinsE John Threlfall
Aug 4, 2021·MSystems·Nicole PearcyTania Dottorini
Aug 30, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J-Y KimB-K Lee
Feb 28, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Apostolos GeorgopoulosWolfgang Graninger