A novel dual ex vivo lung perfusion technique improves immediate outcomes in an experimental model of lung transplantation

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Y TanakaNorihisa Shigemura

Abstract

The lungs are dually perfused by the pulmonary artery and the bronchial arteries. This study aimed to test the feasibility of dual-perfusion techniques with the bronchial artery circulation and pulmonary artery circulation synchronously perfused using ex vivo lung perfusion (EVLP) and evaluate the effects of dual-perfusion on posttransplant lung graft function. Using rat heart-lung blocks, we developed a dual-perfusion EVLP circuit (dual-EVLP), and compared cellular metabolism, expression of inflammatory mediators, and posttransplant graft function in lung allografts maintained with dual-EVLP, standard-EVLP, or cold static preservation. The microvasculature in lung grafts after transplant was objectively evaluated using microcomputed tomography angiography. Lung grafts subjected to dual-EVLP exhibited significantly better lung graft function with reduced proinflammatory profiles and more mitochondrial biogenesis, leading to better posttransplant function and compliance, as compared with standard-EVLP or static cold preservation. Interestingly, lung grafts maintained on dual-EVLP exhibited remarkably increased microvasculature and perfusion as compared with lungs maintained on standard-EVLP. Our results suggest that lung grafts ...Continue Reading

References

Jan 1, 1992·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·L CouraudF Clerc
Jan 1, 1990·The Annals of Thoracic Surgery·H H SchreinemakersJ D Cooper
Oct 1, 1993·The Annals of Thoracic Surgery·G A Patterson
Jan 1, 1996·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·I C WilsonJ H Dark
Jan 22, 1998·The Journal of Thoracic and Cardiovascular Surgery·A VarelaJ Ugarte
Oct 7, 1998·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·M A NørgaardG Pettersson
Jun 29, 1999·The Journal of Thoracic and Cardiovascular Surgery·F VenutaG F Coloni
Apr 12, 2002·World Journal of Surgery·Kim M Olthoff
May 20, 2003·Kidney International·Marilda MazzaliRichard J Johnson
Jul 12, 2003·Anatomy and Embryology·Takao Suzuki, Tatsuo Kasai
Aug 16, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Cynthia M MagroAmy Pope-Harman
May 12, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Heyman LuckrazJohn Wallwork
Sep 16, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Markus KamlerHeinz Jakob
Sep 24, 2004·Journal of Hepatology·Charles BalabaudPaulette Bioulac-Sage
Apr 6, 2006·Advances in Experimental Medicine and Biology·Giuseppe CiccoV Memeo
Sep 26, 2006·The Annals of Thoracic Surgery·Heyman LuckrazJohn Wallwork
Nov 14, 2008·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Walter WederDidier Lardinois
Jan 10, 2009·Proceedings of the American Thoracic Society·Jonathan B Orens, Edward R Garrity
Jan 10, 2009·Proceedings of the American Thoracic Society·Jose Fernando Santacruz, Atul C Mehta
Mar 24, 2009·Translational Research : the Journal of Laboratory and Clinical Medicine·Eric J Grossman, Rebecca A Shilling
Oct 22, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·G EineckeP F Halloran
Mar 27, 2010·American Journal of Respiratory and Critical Care Medicine·Gundeep S DhillonMark R Nicolls
Aug 7, 2010·Current Opinion in Organ Transplantation·Gosta B PetterssonMartin A Nørgaard
Aug 7, 2010·Current Opinion in Organ Transplantation·Ilhan Inci, Walter Weder
Aug 7, 2010·Current Opinion in Organ Transplantation·Mark R Nicolls, Martin R Zamora
Sep 3, 2010·American Journal of Physiology. Renal Physiology·Maarten G SnoeijsL W Ernest van Heurn
Feb 18, 2011·The New England Journal of Medicine·Holger K Eltzschig, Peter Carmeliet
Apr 15, 2011·The New England Journal of Medicine·Marcelo CypelShaf Keshavjee
May 14, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S A LodhiH U Meier-Kriesche
Feb 4, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·N ShigemuraC Bermudez
Feb 14, 2013·Advances in Experimental Medicine and Biology·Mohammad A Khan, Mark R Nicolls
Jul 4, 2013·The Journal of Thoracic and Cardiovascular Surgery·Gösta B PetterssonEugene H Blackstone
Sep 24, 2013·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Roger D YusenUNKNOWN International Society for Heart and Lung Transplantation
Jan 1, 2014·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Geert M VerledenPaul Corris
Jan 17, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Kentaro NodaChristian A Bermudez

❮ Previous
Next ❯

Citations

Dec 31, 2015·Tissue Engineering. Part B, Reviews·Holly Wobma, Gordana Vunjak-Novakovic
Mar 2, 2016·American Journal of Respiratory and Critical Care Medicine·Mark R NicollsNiccolò Daddi
Jul 7, 2016·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Andreas WallinderGöran Dellgren
Feb 28, 2017·Chest·Shinya TaneNorihisa Shigemura
May 3, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Caterina LonatiFranco Valenza
Feb 25, 2017·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Jessica G Y LucJayan Nagendran
Jul 7, 2015·European Journal of Anaesthesiology·Andre Soluri-MartinsPatricia R M Rocco
Mar 24, 2020·The Journal of Thoracic and Cardiovascular Surgery·Ilker IskenderIlhan Inci
Oct 1, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Aizhou WangMarcelo Cypel
Mar 9, 2021·General Thoracic and Cardiovascular Surgery·Daisuke Nakajima, Hiroshi Date
Mar 15, 2020·The Journal of Thoracic and Cardiovascular Surgery·Sarah BurkiJonathan D'Cunha
Sep 7, 2020·The Journal of Thoracic and Cardiovascular Surgery·Samuel J KesseliMatthew G Hartwig

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.