A novel dual-well array chip for efficiently trapping single-cell in large isolated micro-well without complicated accessory equipment

Biomicrofluidics
Chenyu WangYude Yu

Abstract

Conventional cell-sized well arrays have advantages of high occupancy, simple operation, and low cost for capturing single-cells. However, they have insufficient space for including reagents required for cell treatment or analysis, which restricts the wide application of cell-sized well arrays as a single-cell research tool alone. Here, we present a novel dual-well array chip, which integrates capture-wells (20 μm in diameter) with reaction-wells (100 μm in diameter) and describe a flow method for convenient single-cell analysis requiring neither complicated infra-structure nor high expenditure, while enabling highly efficient single cell trapping (75.8%) with only 11.3% multi-cells. Briefly, the cells are first loaded into the dual-wells by gravity and then multi-cells in the reaction-wells are washed out by phosphate buffer saline. Next, biochemical reagents are loaded into reaction-wells using the scraping method and the chip is packed as a sandwich structure. We thereby successfully measured intracellular β-galactosidase activity of K562 cells at the single-cell level. We also used computational simulations to illustrate the working principle of dual-well structure and found out a relationship between the wall shear stress ...Continue Reading

References

Nov 10, 2001·Annual Review of Biomedical Engineering·A Folch, M Toner
Sep 1, 2005·Analytical Chemistry·Jacqueline R Rettig, Albert Folch
Dec 26, 2006·Analytical Chemistry·Dino Di Carlo, Luke P Lee
Feb 17, 2007·Nature Reviews. Microbiology·Douglas B WeibelGeorge M Whitesides
Jul 27, 2007·Lab on a Chip·Mirjam OchsnerMichael L Smith
Nov 1, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yoshiharu TokimitsuAtsushi Muraguchi
Feb 22, 2008·Electrophoresis·Sara LindströmHelene Andersson Svahn
Apr 16, 2008·Analytical Chemistry·Palani KumaresanRichard A Mathies
Nov 1, 2008·Biotechnology and Bioengineering·Natanel KorinShulamit Levenberg
Sep 15, 2009·PloS One·Sara LindströmHelene Andersson-Svahn
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Matthias P LutolfHelen M Blau
Mar 31, 2010·Microfluidics and Nanofluidics·Joong Yull ParkShuichi Takayama
May 11, 2010·Biochimica Et Biophysica Acta·Sara Lindström, Helene Andersson-Svahn
Aug 20, 2010·Lab on a Chip·Ye TianHong-Bo Sun
Aug 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Adam K WhiteCarl L Hansen
Jul 9, 2013·Analytica Chimica Acta·Ying Zhu, Qun Fang
Jan 10, 2014·Biomicrofluidics·A KarimiA M Ardekani
Jan 23, 2015·Biomicrofluidics·D JinW H Wang
Jun 11, 2015·Lab on a Chip·Ching-Hui LinChia-Hsien Hsu
Dec 24, 2015·Biomicrofluidics·Manjima DharDino Di Carlo

❮ Previous
Next ❯

Citations

Jul 11, 2020·Advanced Biosystems·Jacky Fong-Chuen LooYi-Ping Ho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.