A novel electrochemical approach for prolonged measurement of absolute levels of extracellular dopamine in brain slices

ACS Chemical Neuroscience
Mark H BurrellJanusz Lipski

Abstract

Tonic dopamine (DA) levels influence the activity of dopaminergic neurons and the dynamics of fast dopaminergic transmission. Although carbon fiber microelectrodes and fast-scan cyclic voltammetry (FSCV) have been extensively used to quantify stimulus-induced release and uptake of DA in vivo and in vitro, this technique relies on background subtraction and thus cannot provide information about absolute extracellular concentrations. It is also generally not suitable for prolonged (>90 s) recordings due to drift of the background current. A recently reported, modified FSCV approach called fast-scan controlled-adsorption voltammetry (FSCAV) has been used to assess tonic DA levels in solution and in the anesthetized mouse brain. Here we describe a novel extension of FSCAV to investigate pharmacologically induced, slowly occurring changes in tonic (background) extracellular DA concentration, and phasic (stimulated) DA release in brain slices. FSCAV was used to measure adsorption dynamics and changes in DA concentration (for up to 1.5 h, sampling interval 30 s, detection threshold < 10 nM) evoked by drugs affecting DA release and uptake (amphetamine, l-DOPA, pargyline, cocaine, Ro4-1284) in submerged striatal slices obtained from rat...Continue Reading

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Citations

Nov 2, 2017·Journal of Neuroscience Research·Akimasa IshidaHideki Hida
Jul 30, 2020·Analytical Methods : Advancing Methods and Applications·Yangguang OuParastoo Hashemi
Sep 16, 2018·Biosensors & Bioelectronics·Yoonbae OhDong Pyo Jang
May 29, 2018·Analytical Chemistry·Justin A JohnsonR Mark Wightman

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