A novel electrochemical aptamer-antibody sandwich assay for the detection of tau-381 in human serum

The Analyst
Bingqing ShuiZhenzhong Guo

Abstract

Tau protein plays a crucial role in the pathogenesis of Alzheimer's disease (AD). However, the assay to detect low concentrations of tau protein is a great challenge for the early diagnosis of AD. We will outline a novel aptamer-antibody sandwich assay based on an electrochemical biosensor for the detection of tau-381 in human serum. To improve the detection sensitivity, the aptamer-antibody sandwich assay for the detection of tau-381 was developed by using a tau antibody (anti-tau) and an aptamer specific to tau-381 as the recognition element and cysteamine-stabilized gold nanoparticles (AuNPs) for signal amplification. Differential pulse voltammetry (DPV) was employed to record the signal response of tau-381 with different concentrations. The tau-381 concentration ranged from 0.5 pM to 100 pM. The responses of DPV measurements showed excellent results in this dynamic range. This simple, rapid, highly sensitive and specific assay gave a low limit of detection (LOD) of 0.42 pM for tau-381. The feasibility and reliability of the assay were verified by testing tau-381 in human serum from patients with AD. Thus, this method could prove valuable in diagnosing AD within the early stages of the disease.

References

Apr 24, 2003·JAMA : the Journal of the American Medical Association·Trey SunderlandRobert M Cohen
May 14, 2016·Science Translational Medicine·Matthew R BrierBeau M Ances
Nov 11, 2016·Biosensors & Bioelectronics·Scarlet Xiaoyan WangRichard H Bayford
Jan 9, 2018·Biochimie·Bingqing ShuiZhenzhong Guo

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Citations

Jan 9, 2020·Analytical and Bioanalytical Chemistry·Anabel VillalongaReynaldo Villalonga
Jan 5, 2020·Analytical and Bioanalytical Chemistry·Iva ZiuSanela Martic
Nov 27, 2019·Nanomaterials·Pedro CarneiroMaria Carmo Pereira
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Marta Jarczewska, Elżbieta Malinowska
Jan 10, 2021·Biosensors & Bioelectronics·Siddharth Sourabh SahuApurba Dev
Jan 15, 2021·Diagnostics·Ragaa Abd-Ellatief, Maha Ragaa Abd-Ellatief
Nov 26, 2020·Nanotechnology·Divya GopalanSrinivas Mutalik
Nov 7, 2020·ACS Applied Materials & Interfaces·Ling Sum LiuPik Kwan Lo
Feb 20, 2020·Talanta·Pedro CarneiroMaria do Carmo Pereira

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Methods Mentioned

BETA
biosensors
biosensor
transmission electron microscopy
sandwich assay

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