A novel electrochemical aptasensor based on Y-shape structure of dual-aptamer-complementary strand conjugate for ultrasensitive detection of myoglobin

Biosensors & Bioelectronics
Seyed Mohammad TaghdisiKhalil Abnous

Abstract

Monitoring of myoglobin (Mb) in human blood serum is highly in demand for early diagnosis of acute myocardial infarction (AMI). Here, a novel electrochemical aptasensor was developed for ultrasensitive and selective detection of Mb, based on Y-shape structure of dual-aptamer (DApt)-complementary strand of aptamer (CS) conjugate, gold electrode and exonuclease I (Exo I). The designed aptasensor obtains features of gold, such as high electrochemical conductivity and large surface area, property of Y-shape structure of DApt-CS conjugate to function as a gate and obstacle for the access of redox probe to the surface of electrode, as well as high specificity and sensitivity of aptamer toward its target and Exo I as an enzyme which specifically degrades the 3'-end of single-stranded DNA (ssDNA). In the absence of Mb, the Y-shape structure remains intact. So, a weak electrochemical signal is observed. Upon addition of target, the DApt leave the CS and bind to Mb, leading to disassembly of Y-shape structure and following the addition of Exo I, a strong electrochemical signal could be recorded. The fabricated aptasensor showed high selectivity toward Mb with a limit of detection (LOD) as low as 27 pM. Besides, the developed aptasensor w...Continue Reading

References

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Citations

Apr 18, 2016·Biosensors & Bioelectronics·Rezvan Yazdian RobatiSeyed Mohammad Taghdisi
May 24, 2016·Biosensors & Bioelectronics·Seyed Mohammad TaghdisiKhalil Abnous
Jan 9, 2020·Analytical and Bioanalytical Chemistry·Anabel VillalongaReynaldo Villalonga
Apr 20, 2017·Sensors·Yeşeren SaylanAdil Denizli
Sep 14, 2018·Pharmaceuticals·Dario Ruiz CiancioMaría Belén Mestre
Jun 23, 2018·Mikrochimica Acta·Neda Ghafori Nia, Azadeh Azadbakht
Dec 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mahin ShahdordizadehSeyed Mohammad Taghdisi
Jan 15, 2021·Diagnostics·Ragaa Abd-Ellatief, Maha Ragaa Abd-Ellatief
Sep 7, 2019·Biosensors & Bioelectronics·Elham SameiyanSeyed Mohammad Taghdisi

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