A Novel Electrochemical Sensor Based on [Ru(NH3 )6 ]Cl3 as a Redox Indicator for the Detection of G-G Mismatched DNA

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Xiaoqian ZhuShengfu Wang

Abstract

In this paper, a novel electrochemical sensor was developed for the rapid detection of G-G mismatched DNA based on hexaammineruthenium(III) chloride ([Ru(NH3)6]Cl3) as a redox indicator. The sensor platform was constructed by immobilizing small molecules (NC-linker) on the gold electrode via amide bonds. The as-prepared NC-linker as the nucleic acids recognition molecule can interact with the G base of DNA. After the sensor was incubated with G-G mismatched DNA, the double-stranded DNA (dsDNA) acted as carriers of the signal tags-[Ru(NH3)6]Cl3, which resulted in a remarkable electrochemical signal. More binding of [Ru(NH3)6]Cl3 led to increases of the electrochemical signal. Other mismatched DNA produced only a low response, as well as complementary DNA. Thus G-G mismatched DNA can be easily discriminated from other mismatched and complementary DNA based on the sensor. Furthermore, the method was simple, rapid and repeatable for the detection of G-G mismatched DNA. The selective detection of target dsDNA was achieved by a relative current ratio of the target and control DNA. These results demonstrated that this strategy could provide great promise for the rapid and specific detection of other sequence-specific DNA.

References

Mar 8, 2008·Journal of the American Chemical Society·Elena E FerapontovaKurt V Gothelf
Mar 6, 2010·Accounts of Chemical Research·Arica A Lubin, Kevin W Plaxco
Oct 31, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Elaheh FarjamiElena E Ferapontova
Feb 7, 2013·Physical Review Letters·M AblikimUNKNOWN BESIII Collaboration
Dec 17, 2015·Chemical Communications : Chem Comm·Ye TengErkang Wang

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Citations

Jan 5, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaojing WangChangjun Hou

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