A Novel Electrochemiluminescent Immunoassay Based on Target Transformation Assisted with Catalyzed Hairpin Assembly Amplification for the Ultrasensitive Bioassay

ACS Applied Materials & Interfaces
Hu-Xin LeiPu Liao

Abstract

In this work, we constructed a novel electrochemiluminescent (ECL) strategy based on sandwich immunoassay-induced target transformation assisted with catalyzed hairpin assembly (CHA) amplification for ultrasensitive bioassay with cysteine-rich protein 61 (CCN1) as a model. First, the target CCN1 could be equally transformed into the specific oligonucleotide (initiator I) labeled on the detection antibody based on the specific sandwich immunoassay. In addition, the initiator I triggered an efficient nonenzymatic CHA amplification in the presence of ferrocene-labeled hairpin 1 (Fc-H1) and hairpin 2 (H2) to produce massive hybrids (Fc-H1-H2) containing a sticky end labeled with ferrocene. Finally, Fc-H1-H2 could be immobilized on the capture probe single-stranded DNA (ssDNA)-modified electrode through the hybridization between the sticky end of Fc-H1-H2 and ssDNA, and a significantly quenched ECL signal could be obtained due to the efficient quench effect between ferrocene and the ECL indicator, ruthenium(II) tris(4,4'-dicarboxylicacid-2,2'-bipyridyl) [Ru(dcbpy)32+], immobilized on the surface of the electrode, which was related to the concentration of target CCN1. As expected, the proposed ECL biosensor exhibited a relatively low...Continue Reading

References

Jun 14, 2012·Cell Biochemistry and Function·Jana JandovaGeorge S Watts
Aug 17, 2012·Journal of the American Chemical Society·Bingling LiAndrew D Ellington
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xi ChenAndrew D Ellington
Oct 5, 2013·Journal of Pharmaceutical and Biomedical Analysis·Emily G Armitage, Coral Barbas
Dec 29, 2013·The Journal of Biological Chemistry·Daniel Dongwei WuMei-Zhen Cui
Sep 5, 2014·Cancer Cell International·Chang-Chun NiuWei-Ke Si
Dec 17, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gang ChengJianning Zhang
Mar 4, 2015·British Journal of Cancer·S McPhailB Rous
May 20, 2015·Oncotarget·María Pilar Sánchez-BailónJorge Martín-Pérez
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Oct 16, 2016·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y F SongQ Lin
Jul 2, 2017·Breast Cancer Research : BCR·Jan PoleszczukHeiko Enderling
Aug 31, 2017·Molecular Cancer·Weihua LiDexi Chen
Jun 27, 2018·Journal of the American Chemical Society·Aruni P K K Karunanayake MudiyanselageMingxu You

❮ Previous
Next ❯

Citations

Feb 19, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rashaad A HusainZong-Hong Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.