A novel electrochemiluminescent immunosensor based on the quenching effect of aminated graphene on nitrogen-doped carbon quantum dots

Analytica Chimica Acta
Jing ZhouQin Wei

Abstract

Nitrogen-doped carbon quantum dots (N-CQDs) with an average diameter of 2 nm were synthesized by carbonization of diethylene triamine pentacetate acid (DTPA). The simple prepared N-CQDs showed excellent electrochemiluminescence (ECL) property and were used as luminophors to fabricate a sandwich-type ECL immunosensor. Aminated graphene (NH2-G) was also synthesized and used as a label of secondary antibody. The labeled NH2-G could effectively quench the ECL of N-CQDs modified on electrodes due to ECL resonance energy transfer (ERET). Immunological recognition which induced ECL quenching enabled the quantitative determination of biomarkers. Alpha fetoprotein (AFP) was selected as a model analyte to investigate the analytical performance of the proposed immunosensor. Under optimal conditions, a good linear relationship between ECL intensity and the logarithm of AFP concentration was obtained in the range of 0.01-100 ng mL(-1) with the detection limit of 3.3 pg mL(-1). The proposed ECL immunosensor showed good stability, acceptable selectivity and reproducibility.

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Citations

Jun 5, 2016·Talanta·Li-Ming Shen, Jing Liu
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Mar 25, 2020·Environmental Chemistry Letters·Siavash Iravani, Rajender S Varma
Nov 9, 2017·Chemical Society Reviews·Yuanhong XuShaojun Guo
Jul 4, 2019·ACS Sensors·Meixiu LiJingquan Liu

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