PMID: 8445254Feb 26, 1993Paper

A novel ELISA assay for the detection of C3 nephritic factor

Journal of Immunological Methods
J SeinoM R Daha

Abstract

We have developed an ELISA procedure for the detection of C3 nephritic factor (C3NeF), in which wells are coated with a fixed concentration of 2 micrograms C3b per well, and subsequently reacted with B and D. The presence of increasing concentrations of NiCl2 showed a NiCl2 concentration-dependent generation of C3bBb and very little solid-phase bound C3bBb was generated with MgCl2. The formation of solid-phase C3bBb in the presence of an optimal concentration of 1 mM NiCl2, was time-dependent and plateau values were reached after 30 min at 37 degrees C. IgG purified from the serum of a patient with membranoproliferative glomerulonephritis (MPGN) type II containing C3NeF stabilizing activity was bound to the C3bBb generated on microwells in a dose-dependent manner whereas normal IgG exhibited only minor reactivity. C3NeF activity was measured using the ELISA method in patients with MPGN type II (n = 15) and other diseases (n = 17) and in normal controls (n = 15). Most of the patients with MPGN type II exhibited positive C3NeF at various levels, while two of the disease controls showed only slight reactivities. C3NeF titers measured by this new ELISA procedure correlated well with previously described hemolytic assays (r = 0.617,...Continue Reading

References

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Citations

Apr 26, 2013·Immunologic Research·Eleonora BallantiRoberto Perricone
Apr 9, 2001·Journal of Immunological Methods·E JelezarovaH U Lutz
Jan 10, 2012·Clinical Journal of the American Society of Nephrology : CJASN·Yuzhou ZhangRichard J H Smith
Jan 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Simone A JoostenLeendert C Paul
Oct 16, 2008·The Journal of Clinical Endocrinology and Metabolism·David B SavageStephen O'Rahilly
May 10, 2019·Frontiers in Immunology·Fernando CorvilloMargarita López-Trascasa
Nov 9, 2018·Frontiers in Immunology·Kristina N EkdahlBo Nilsson

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