A novel ELISA-based diagnosis of acquired von Willebrand disease with increased VWF proteolysis

Thrombosis and Haemostasis
Antoine RauchSophie Susen

Abstract

Von Willebrand disease-type 2A (VWD-2A) and acquired von Willebrand syndrome (AVWS) due to aortic stenosis (AS) or left ventricular assist device (LVAD) are associated with an increased proteolysis of von Willebrand factor (VWF). Analysis of VWF multimeric profile is the most sensitive way to assess such increased VWF-proteolysis. However, several technical aspects hamper a large diffusion among routine diagnosis laboratories. This makes early diagnosis and early appropriate care of increased proteolysis challenging. In this context of unmet medical need, we developed a new ELISA aiming a quick, easy and reliable assessment of VWF-proteolysis. This ELISA was assessed successively in a LVAD-model, healthy subjects (n=39), acquired TTP-patients (n=4), VWD-patients (including VWD-2A(IIA), n=22; VWD-2B, n=26; VWD-2A(IIE), n=21; and VWD-1C, n=8) and in AVWS-patients (AS, n=9; LVAD, n=9; and MGUS, n=8). A standard of VWF-proteolysis was specifically developed. Extent of VWF-proteolysis was expressed as relative percentage and as VWF proteolysis/VWF:Ag ratio. A speed-dependent increase in VWF-proteolysis was assessed in the LVAD model whereas no proteolysis was observed in TTP-patients. In VWD-patients, VWF-proteolysis was significant...Continue Reading

References

Jul 25, 2003·The New England Journal of Medicine·André VincentelliBrigitte Jude
Dec 3, 2005·Blood·Wolf Achim HassenpflugReinhard Schneppenheim
Jan 22, 2009·The Journal of Thoracic and Cardiovascular Surgery·Sheri CrowLyle Joyce
Mar 31, 2010·Thrombosis and Haemostasis·Claudia HeilmannBarbara Zieger
May 2, 2012·Blood·Marie Moatti-CohenUNKNOWN French Reference Center for Thrombotic Microangiopathies
Oct 31, 2012·Journal of Thrombosis and Haemostasis : JTH·G OcakW M Lijfering
Nov 16, 2012·The New England Journal of Medicine·Joseph Loscalzo
Jan 29, 2013·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·David FeldmanUNKNOWN International Society for Heart and Lung Transplantation
Sep 3, 2013·Journal of the American College of Cardiology·Matthew C BunteMaria M Mountis
Nov 29, 2013·The New England Journal of Medicine·Randall C StarlingNicholas G Smedira
Mar 20, 2014·The American Journal of Gastroenterology·Christian S Jackson, Lauren B Gerson
Jun 15, 2014·Blood·Timothy A Springer
Jul 18, 2014·Thrombosis and Haemostasis·Antoine RauchPeter J Lenting
Sep 25, 2014·Journal of Thrombosis and Haemostasis : JTH·J L BlackshearD Chen
Mar 17, 2015·JACC. Heart Failure·Daniel J GoldsteinUNKNOWN ADVANCE Investigators
Oct 13, 2015·British Journal of Haematology·Alessandra CasonatoLuigi De Marco

❮ Previous
Next ❯

Citations

Jul 1, 2011·Journal of Pediatric and Adolescent Gynecology·Nancy A SokkaryLakshmi Venkateswaran
Apr 1, 2019·Journal of Thrombosis and Haemostasis : JTH·Masaki HayakawaMasanori Matsumoto
Dec 6, 2017·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Joerg KellermairClemens Steinwender
Aug 2, 2016·Journal of Atherosclerosis and Thrombosis·Koichi Kokame
Jun 8, 2018·Haemophilia : the Official Journal of the World Federation of Hemophilia·F W G Leebeek, S Susen
Jun 28, 2017·World Journal of Gastroenterology : WJG·Grigoriy E Gurvits, Elena Fradkov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Heart & Ventricular Assist Devices

Total artificial hearts (TAH) and ventricular assist devices (VADs) provide cardiac support for patients with end-stage heart disease and have significantly improved the survival of these patients. Discover the latest research on Artificial Heart and Ventricular Assist Devices here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
A G. RoseL W. Miller
Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association
Frank Czul, Jamie S Barkin
The Journal of Thoracic and Cardiovascular Surgery
Karine NubretAlexandre Ouattara
© 2022 Meta ULC. All rights reserved