A novel enzyme immunoassay for conidia of Fusarium oxysporum f.sp. cucumerinum F504

Microbiology and Immunology
T KitagawaH Ogura

Abstract

This study was undertaken to develop a new tool to study fusarial diseases of plants. Micro- and macro-conidia of a strain (F504) of Fusarium oxysporum were isolated and antiserum against the conidia was elicited in rabbits. A highly specific and sensitive competitive-type enzyme-linked immunosorbent assay (ELISA) for conidia of the strain was developed using the antiserum with beta-D-galactosidase-labeled anti-rabbit IgG as the secondary antibody and conidia fragments of the strain as antigen attached to Amino-Dylark solid-phase balls. The assay was highly specific to conidia of the strain F504, while conidia-free hypha of the strain F504 as well as all other microorganisms tested including nine other strains of Fusarium species showed little cross-reactivity. Application of the ELISA to following the growth rates of conidia in hyphae of the strain F504 under several conditions are also reported.

References

Jan 1, 1986·Advances in Immunity and Cancer Therapy·K Fujiwara, T Kitagawa
Jan 1, 1987·Microbiology and Immunology·T KitagawaS Mogi
Nov 7, 1985·Journal of Immunological Methods·H TanimoriT Kitagawa
Sep 1, 1981·The Journal of Antibiotics·H SuzukiT Kitagawa

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