A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66T Encoded in a Sizeable Polysaccharide Utilization Locus

Frontiers in Microbiology
Mikkel Schultz-JohansenPeter Stougaard

Abstract

Marine microbes are a rich source of enzymes for the degradation of diverse polysaccharides. Paraglaciecola hydrolytica S66T is a marine bacterium capable of hydrolyzing polysaccharides found in the cell wall of red macroalgae. In this study, we applied an approach combining genomic mining with functional analysis to uncover the potential of this bacterium to produce enzymes for the hydrolysis of complex marine polysaccharides. A special feature of P. hydrolytica S66T is the presence of a large genomic region harboring an array of carbohydrate-active enzymes (CAZymes) notably agarases and carrageenases. Based on a first functional characterization combined with a comparative sequence analysis, we confirmed the enzymatic activities of several enzymes required for red algal polysaccharide degradation by the bacterium. In particular, we report for the first time, the discovery of novel enzyme activities targeting furcellaran, a hybrid carrageenan containing both β-carrageenan and κ/β-carrageenan motifs. Some of these enzymes represent a new subfamily within the CAZy classification. From the combined analyses, we propose models for the complete degradation of agar and κ/β-type carrageenan by P. hydrolytica S66T. The novel enzymes d...Continue Reading

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Citations

May 31, 2019·Marine Drugs·Maria Dolores TorresHerminia Domínguez
Jan 12, 2020·Applied and Environmental Microbiology·Sora YuKyoung Heon Kim
Jun 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erna LiDaorui Pang
Dec 31, 2019·Communications Biology·Andrew G HettleAlisdair B Boraston
Sep 12, 2020·International Journal of Environmental Research and Public Health·Sara García-PozaAna M M Gonçalves
Sep 11, 2019·The Journal of Biological Chemistry·Alexander Holm ViborgHarry Brumer
Jan 15, 2020·Journal of Agricultural and Food Chemistry·Rosanna C HennessyPeter Stougaard
Nov 11, 2020·Journal of Bacteriology·Robert W P Glowacki, Eric C Martens
Mar 1, 2021·FEMS Microbiology Letters·Josefine HansenEva C Sonnenschein
Apr 24, 2021·Chembiochem : a European Journal of Chemical Biology·Marcus BäumgenUwe T Bornscheuer
Jun 11, 2020·Journal of Agricultural and Food Chemistry·Yuying ZhangChanghu Xue
Aug 21, 2021·FEMS Microbiology Ecology·Cheng XueDa-Zhi Wang

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Methods Mentioned

BETA
PCR
PCRs
electrophoresis
Reverse Transcription PCR
Assay

Software Mentioned

dbCAN
BLASTp
MEGA6
LipoP
MaGe interface
SulfAtlas
RAST
CLC ( Main Workbench )
SignalP
MicroScope

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