A novel epitope tag for the detection of rabGTPases

Electrophoresis
B NagelkerkenP van der Sluijs

Abstract

Rab GTPases are localized on the cytoplasmic surface of most intracellular organelles where they play a role in the regulation of vesicular transport. As it has been difficult to detect endogenous rab proteins by morphological methods, their localizations were often inferred from transfection experiments using epitope-tagged constructs. Because most of the available epitope tags are only recognitzed by mouse monoclonal antibodies they are often not suitable for double or triple label immunocytochemistry. To overcome this problem, we generated antibodies against a novel 10 amino acid X31 influenza hemagglutin epitope (NH). We here characterized these antibodies and document their utility for detecting early endosomal rab proteins N-terminally tagged with the NH decapeptide in morphological and biochemical assays.

References

Mar 8, 1991·Cell·J P GorvelJ Gruenberg
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P Van Der SluijsI Mellman
Dec 1, 1985·Molecular and Cellular Biology·G I EvanJ M Bishop
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y T ChenH P Moore
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·E DaroI Mellman
Nov 15, 1996·The Journal of Biological Chemistry·G BottgerP van der Sluijs
Nov 1, 1996·The Journal of Cell Biology·O UllrichR G Parton
May 5, 1997·The Journal of Cell Biology·G JeddN Segev
Sep 23, 1997·Cytometry·P ChavrierJ P Gorvel

❮ Previous
Next ❯

Citations

Mar 22, 2002·Cellular Microbiology·Bruno RobibaroKeith A Joiner
Dec 25, 2009·Scandinavian Journal of Caring Sciences·Maria Afrell, Carl Edvard Rudebeck
Jan 16, 2002·The Journal of Biological Chemistry·Karin MohrmannPeter van Der Sluijs
Jul 31, 1999·The Journal of Biological Chemistry·P A Zuk, L A Elferink
May 20, 1999·Molecular Membrane Biology·K Mohrmann, P van der Sluijs
May 31, 2002·The Journal of Biological Chemistry·Karin MohrmannPeter van der Sluijs

❮ Previous
Next ❯

Related Concepts

Related Feeds