A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China.

PloS One
Yanwen XiongJian-guo Xu

Abstract

An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.

References

Mar 24, 1983·The New England Journal of Medicine·L W RileyM L Cohen
Nov 5, 1998·The Pediatric Infectious Disease Journal·F ProulxE Seidman
Oct 26, 1999·Infection and Immunity·C M ThorpeD W Acheson
Nov 5, 1999·The American Journal of Tropical Medicine and Hygiene·M KobayashiN Agui
Dec 22, 1999·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·C LitalienE Seidman
Nov 28, 2002·Journal of Clinical Microbiology·Marjut EklundAnja Siitonen
Oct 16, 2003·Biochemical and Biophysical Research Communications·Saori NakagawaTatsuo Yamamoto
Jul 3, 2004·Genome Research·Aaron C E DarlingNicole T Perna
Mar 5, 2005·Molecular Biology and Evolution·A Carolin FrankSiv G E Andersson
Mar 23, 2005·Lancet·Phillip I TarrWayne L Chandler
Nov 7, 2006·Journal of Animal Science·C L Gyles
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jan 26, 2008·Emerging Infectious Diseases·Peter C H FengThomas S Whittam
Mar 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shannon D ManningThomas S Whittam
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Oct 2, 2008·Emerging Infectious Diseases·Juliana GrantRobert T Rolfs
Dec 4, 2009·Journal of Clinical Microbiology·Changyun YeJianguo Xu
Dec 2, 2010·Foodborne Pathogens and Disease·Witold A Ferens, Carolyn J Hovde
Feb 25, 2011·Journal of Clinical Microbiology·Ping WangJianguo Xu
Mar 23, 2011·Applied and Environmental Microbiology·Mark EppingerThomas A Cebula
Jun 24, 2011·The New England Journal of Medicine·Christina FrankUNKNOWN HUS Investigation Team
Jul 29, 2011·The New England Journal of Medicine·David A RaskoMatthew K Waldor
Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mark EppingerThomas A Cebula

❮ Previous
Next ❯

Citations

Apr 17, 2013·International Journal of Food Microbiology·Adrian Canizalez-RomanNidia León-Sicairos
Aug 21, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Mei Ling ChenJian Ling Jin
Mar 19, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Qiong MengJianguo Xu
Jul 23, 2016·Frontiers in Microbiology·Brigida RusconiMark Eppinger
Jun 30, 2016·Microbial Pathogenesis·Lirong WangAijun Lv
Dec 18, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mohamed A Karmali
Jan 6, 2018·International Journal of Environmental Research and Public Health·Yan ChenYong-Ning Wu
Jan 30, 2020·Microorganisms·Adriene Maria Ferreira CavalcantiLuís Fernando Dos Santos
May 29, 2016·Environmental Science and Pollution Research International·Nawal Nouridaim AbuelhassanWan Mohtar Yusoff
Apr 8, 2020·BMC Medical Genomics·Le TangShu-Lin Liu
Nov 18, 2016·Frontiers in Cellular and Infection Microbiology·Xiangning BaiYanwen Xiong
Apr 12, 2019·Toxins·Moo-Seung Lee, Vernon L Tesh
Nov 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Vinicius Silva CastroEduardo Eustáquio Souza Figuiredo

❮ Previous
Next ❯

Datasets Mentioned

BETA
EDL933
CP001925
CP001926

Methods Mentioned

BETA
PCR

Software Mentioned

MEGA
TraE
BLASTN
eBURST
EcMLST

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.