A novel esterase subfamily with α/β-hydrolase fold suggested by structures of two bacterial enzymes homologous to L-homoserine O-acetyl transferases

FEBS Letters
Christine TölzerKarsten Niefind

Abstract

MekB from Pseudomonas veronii and CgHle from Corynebacteriumglutamicum belong to the superfamily of α/β-hydrolase fold proteins. Based on sequence comparisons, they are annotated as homoserine transacetylases in popular databases like UNIPROT, PFAM or ESTHER. However, experimentally, MekB and CgHle were shown to be esterases that hydrolyse preferentially acetic acid esters. We describe the x-ray structures of these enzymes solved to high resolution. The overall structures confirm the close relatedness to experimentally validated homoserine acetyl transferases, but simultaneously the structures exclude the ability of MekB and CgHle to bind homoserine and acetyl-CoA. Insofar the MekB and CgHle structures suggest dividing the homoserine transacetylase family into subfamilies, namely genuine acetyl transferases and acetyl esterases with MekB and CgHle as constituting members of the latter.

References

Apr 1, 1992·Protein Engineering·D L OllisJ Schrag
Apr 13, 2001·Bioinformatics·C Gille, C Frömmel
Jan 23, 2003·Bioinformatics·Charles S Bond
Mar 14, 2007·Journal of Bacteriology·Christina OnacaJosef Altenbuchner
Mar 14, 2007·Antimicrobial Agents and Chemotherapy·Ishac NaziGerard D Wright
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Oct 12, 2007·Biochemical and Biophysical Research Communications·Mingzhu WangWeimin Gong
Dec 14, 2007·Nucleic Acids Research·Minoru KanehisaYoshihiro Yamanishi
Jan 20, 2009·Bioinformatics·Andrew M WaterhouseGeoffrey J Barton
Jan 21, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Christine TölzerKarsten Niefind
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Mar 20, 2010·Annual Review of Biochemistry·Olga Khersonsky, Dan S Tawfik
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 28, 2011·The Journal of Antibiotics·Gianfranco De PascaleGerard D Wright
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans, Garib N Murshudov
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
May 13, 2014·Nucleic Acids Research·Christoph GilleAndreas Gille
Oct 8, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Bharani ThangaveluRonald Viola
Oct 29, 2014·Nucleic Acids Research·UNKNOWN UniProt Consortium

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